Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9553 | 3' | -49.3 | NC_002551.1 | + | 6495 | 0.68 | 0.368149 |
Target: 5'- uCGAuGGGCuCAaCGCCugGu--GGGACu -3' miRNA: 3'- -GCUuUCCG-GUaGCGGugUuuuUCCUG- -5' |
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9553 | 3' | -49.3 | NC_002551.1 | + | 387 | 0.69 | 0.290689 |
Target: 5'- aCGAauccAAGGCUGUCGCCuucucccuuccCAAAAcGGACg -3' miRNA: 3'- -GCU----UUCCGGUAGCGGu----------GUUUUuCCUG- -5' |
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9553 | 3' | -49.3 | NC_002551.1 | + | 1385 | 0.7 | 0.265118 |
Target: 5'- -cAGGGGCCAUC-CCACAuaauGGGAa -3' miRNA: 3'- gcUUUCCGGUAGcGGUGUuuu-UCCUg -5' |
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9553 | 3' | -49.3 | NC_002551.1 | + | 1734 | 1.12 | 0.000175 |
Target: 5'- gCGAAAGGCCAUCGCCACAAAAAGGACa -3' miRNA: 3'- -GCUUUCCGGUAGCGGUGUUUUUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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