Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 956 | 3' | -52.8 | NC_000902.1 | + | 45887 | 0.66 | 0.811759 |
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Target: 5'- uGCGGCAGgugcauaugCGAUGAuaaggcugGCgGUAGCGGAg -3' miRNA: 3'- uUGCCGUCg--------GCUAUU--------UGgUAUCGCCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 31134 | 0.67 | 0.750745 |
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Target: 5'- aGGCGGUAGCUGuggcAUCcgGGCGGGu -3' miRNA: 3'- -UUGCCGUCGGCuauuUGGuaUCGCCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 52887 | 0.68 | 0.740061 |
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Target: 5'- uGGCGGagaaCAGCCGaAUAAAUuCGUGGCGGc -3' miRNA: 3'- -UUGCC----GUCGGC-UAUUUG-GUAUCGCCu -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 15941 | 0.69 | 0.673984 |
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Target: 5'- uGCGGCGGCUGAaaaaugGAACCGgAGacaGGAg -3' miRNA: 3'- uUGCCGUCGGCUa-----UUUGGUaUCg--CCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 56578 | 0.69 | 0.640222 |
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Target: 5'- aAAUGGCAgcguauuuGCUGAaAAAgCAUGGCGGAu -3' miRNA: 3'- -UUGCCGU--------CGGCUaUUUgGUAUCGCCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 54023 | 0.71 | 0.561642 |
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Target: 5'- gGACGGCGGuUCGGggauGACCAacGCGGAa -3' miRNA: 3'- -UUGCCGUC-GGCUau--UUGGUauCGCCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 54781 | 0.76 | 0.287381 |
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Target: 5'- aGACGGCGG-UGAUGAACCugaaGGCGGAg -3' miRNA: 3'- -UUGCCGUCgGCUAUUUGGua--UCGCCU- -5' |
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| 956 | 3' | -52.8 | NC_000902.1 | + | 25592 | 1.07 | 0.002211 |
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Target: 5'- gAACGGCAGCCGAUAAACCAUAGCGGAg -3' miRNA: 3'- -UUGCCGUCGGCUAUUUGGUAUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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