Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 958 | 3' | -54.5 | NC_000902.1 | + | 12183 | 0.66 | 0.761545 |
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Target: 5'- -cAUCuuUGCCagaCCGCGcugGGUAAGCGUc -3' miRNA: 3'- aaUAGcgACGGg--GGCGC---UUAUUCGCG- -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 16637 | 0.68 | 0.642102 |
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Target: 5'- -cGUCGUcgGCCUCaguuaGCcAGUGAGCGCa -3' miRNA: 3'- aaUAGCGa-CGGGGg----CGcUUAUUCGCG- -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 36384 | 0.7 | 0.531913 |
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Target: 5'- --uUCGCUgGCCCCUGCagGAAUu-GUGCg -3' miRNA: 3'- aauAGCGA-CGGGGGCG--CUUAuuCGCG- -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 27191 | 0.71 | 0.479238 |
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Target: 5'- aUcgCGCUGCgCUuuGUaGGUGAGCGCg -3' miRNA: 3'- aAuaGCGACG-GGggCGcUUAUUCGCG- -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 32346 | 0.73 | 0.356075 |
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Target: 5'- ---aCGCUGCCCCUGUGucaGGGCGa -3' miRNA: 3'- aauaGCGACGGGGGCGCuuaUUCGCg -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 23938 | 0.8 | 0.119454 |
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Target: 5'- cUGUCGaugaUGCCCCCGCGuuu-AGCGCc -3' miRNA: 3'- aAUAGCg---ACGGGGGCGCuuauUCGCG- -5' |
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| 958 | 3' | -54.5 | NC_000902.1 | + | 32582 | 1.1 | 0.000971 |
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Target: 5'- cUUAUCGCUGCCCCCGCGAAUAAGCGCa -3' miRNA: 3'- -AAUAGCGACGGGGGCGCUUAUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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