Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9587 | 3' | -52.1 | NC_002577.1 | + | 121790 | 0.66 | 0.990631 |
Target: 5'- aCGAggcgCaCGcGCGCCUGCUC-CGGCg -3' miRNA: 3'- -GCUaaaaGaGC-UGCGGAUGGGaGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 11908 | 0.66 | 0.990631 |
Target: 5'- aCGAggcgCaCGcGCGCCUGCUC-CGGCg -3' miRNA: 3'- -GCUaaaaGaGC-UGCGGAUGGGaGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 109933 | 0.66 | 0.986335 |
Target: 5'- gCGAUgggUCUCGACGaCCgcgucgaGCCCagGGg -3' miRNA: 3'- -GCUAaa-AGAGCUGC-GGa------UGGGagCCg -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 122886 | 0.67 | 0.978437 |
Target: 5'- cCGAg---CUCGGCcaGUUcgaUGCCCUCGGUa -3' miRNA: 3'- -GCUaaaaGAGCUG--CGG---AUGGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 10728 | 0.67 | 0.978437 |
Target: 5'- cCGAg---CUCGGCcaGUUcgaUGCCCUCGGUa -3' miRNA: 3'- -GCUaaaaGAGCUG--CGG---AUGGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 5051 | 0.68 | 0.973409 |
Target: 5'- cCGAgcaUCggcgCGcGCGCCgugucccCCCUCGGCg -3' miRNA: 3'- -GCUaaaAGa---GC-UGCGGau-----GGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 139729 | 0.68 | 0.973409 |
Target: 5'- -cAUUUUgUCGGCGCUccuucgcuUCCUCGGCc -3' miRNA: 3'- gcUAAAAgAGCUGCGGau------GGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 157609 | 0.68 | 0.973409 |
Target: 5'- -cAUUUUgUCGGCGCUccuucgcuUCCUCGGCc -3' miRNA: 3'- gcUAAAAgAGCUGCGGau------GGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 128563 | 0.68 | 0.973409 |
Target: 5'- cCGAgcaUCggcgCGcGCGCCgugucccCCCUCGGCg -3' miRNA: 3'- -GCUaaaAGa---GC-UGCGGau-----GGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 129179 | 0.68 | 0.967583 |
Target: 5'- ------aCUCGGCGCgCgUACCCgCGGCc -3' miRNA: 3'- gcuaaaaGAGCUGCG-G-AUGGGaGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 160615 | 0.68 | 0.967583 |
Target: 5'- ------cCUCGACGCCUAUUgUCcaGGCg -3' miRNA: 3'- gcuaaaaGAGCUGCGGAUGGgAG--CCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 4435 | 0.68 | 0.967583 |
Target: 5'- ------aCUCGGCGCgCgUACCCgCGGCc -3' miRNA: 3'- gcuaaaaGAGCUGCG-G-AUGGGaGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 136723 | 0.68 | 0.967583 |
Target: 5'- ------cCUCGACGCCUAUUgUCcaGGCg -3' miRNA: 3'- gcuaaaaGAGCUGCGGAUGGgAG--CCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 99973 | 0.68 | 0.964019 |
Target: 5'- aGGUUcUCUCGAacuuCGUUUGgaccggaauuauuCCCUCGGCa -3' miRNA: 3'- gCUAAaAGAGCU----GCGGAU-------------GGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 146605 | 0.68 | 0.964019 |
Target: 5'- --cUUUUCUCGgcgguuuggcaaaACGCC--CCCUUGGCa -3' miRNA: 3'- gcuAAAAGAGC-------------UGCGGauGGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 10584 | 0.69 | 0.938788 |
Target: 5'- gGAUgaUCUCgGACGCgcgucgguccguuuCUGCCCUugCGGCg -3' miRNA: 3'- gCUAaaAGAG-CUGCG--------------GAUGGGA--GCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 123029 | 0.7 | 0.917634 |
Target: 5'- gGAUgagUCUCgGACGCgcgucgguccguuuCUGCCCUugCGGCg -3' miRNA: 3'- gCUAaa-AGAG-CUGCG--------------GAUGGGA--GCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 95744 | 0.71 | 0.89462 |
Target: 5'- cCGGUUcuggggUCUCGAUGCCgugaACUCaagUCGGCg -3' miRNA: 3'- -GCUAAa-----AGAGCUGCGGa---UGGG---AGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 2862 | 0.92 | 0.092312 |
Target: 5'- gCGAUUUcCUCuGCGCCUACCCUCGGCc -3' miRNA: 3'- -GCUAAAaGAGcUGCGGAUGGGAGCCG- -5' |
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9587 | 3' | -52.1 | NC_002577.1 | + | 130752 | 0.92 | 0.092312 |
Target: 5'- gCGAUUUcCUCuGCGCCUACCCUCGGCc -3' miRNA: 3'- -GCUAAAaGAGcUGCGGAUGGGAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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