Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9588 | 5' | -57.2 | NC_002577.1 | + | 159855 | 0.76 | 0.35546 |
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Target: 5'- --aCCGuCCCCGGcagGGAuGUCCGGACGCg -3' miRNA: 3'- uucGGC-GGGGCCa--UUU-CAGGUCUGCG- -5' |
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| 9588 | 5' | -57.2 | NC_002577.1 | + | 137483 | 0.76 | 0.35546 |
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Target: 5'- --aCCGuCCCCGGcagGGAuGUCCGGACGCg -3' miRNA: 3'- uucGGC-GGGGCCa--UUU-CAGGUCUGCG- -5' |
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| 9588 | 5' | -57.2 | NC_002577.1 | + | 8167 | 0.76 | 0.347699 |
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Target: 5'- aAGGCCGUacggacaCCGGUGc-GUCCGGGCGCu -3' miRNA: 3'- -UUCGGCGg------GGCCAUuuCAGGUCUGCG- -5' |
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| 9588 | 5' | -57.2 | NC_002577.1 | + | 125446 | 0.76 | 0.347699 |
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Target: 5'- aAGGCCGUacggacaCCGGUGc-GUCCGGGCGCu -3' miRNA: 3'- -UUCGGCGg------GGCCAUuuCAGGUCUGCG- -5' |
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| 9588 | 5' | -57.2 | NC_002577.1 | + | 3343 | 1.1 | 0.001952 |
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Target: 5'- gAAGCCGCCCCGGUAAAGUCCAGACGCg -3' miRNA: 3'- -UUCGGCGGGGCCAUUUCAGGUCUGCG- -5' |
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| 9588 | 5' | -57.2 | NC_002577.1 | + | 130271 | 1.1 | 0.001952 |
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Target: 5'- gAAGCCGCCCCGGUAAAGUCCAGACGCg -3' miRNA: 3'- -UUCGGCGGGGCCAUUUCAGGUCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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