miRNA: 3'- aGGAGGAAGCGccCCACGAaCUCUag -5'
miRNA: 3'- aGGAGGAAGCGccCCACGAaCUCUag -5'
miRNA: 3'- -AGG-AGGAAgCGCcCCACGaACUCUAG- -5'
miRNA: 3'- -AGGAGGAAGCGCCCCACGAACUCUAG- -5'
miRNA: 3'- -AGGAGGAAGCGCCCCACGAACUCUAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9589 | 3' | -55.9 | NC_002577.1 | + | 3308 | 0.66 | 0.902276 |
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Target: 5'- cCCUCCUUCGUccGGUGUUcGAGGc- -3' miRNA: 3'- aGGAGGAAGCGccCCACGAaCUCUag -5' |
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| 9589 | 3' | -55.9 | NC_002577.1 | + | 130306 | 0.66 | 0.902276 |
|
Target: 5'- cCCUCCUUCGUccGGUGUUcGAGGc- -3' miRNA: 3'- aGGAGGAAGCGccCCACGAaCUCUag -5' |
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| 9589 | 3' | -55.9 | NC_002577.1 | + | 4879 | 0.68 | 0.81089 |
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Target: 5'- cUCCgUCCUUaGCGuGGUGCcUGAGAUUu -3' miRNA: 3'- -AGG-AGGAAgCGCcCCACGaACUCUAG- -5' |
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| 9589 | 3' | -55.9 | NC_002577.1 | + | 4741 | 1.11 | 0.00234 |
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Target: 5'- uUCCUCCUUCGCGGGGUGCUUGAGAUCa -3' miRNA: 3'- -AGGAGGAAGCGCCCCACGAACUCUAG- -5' |
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| 9589 | 3' | -55.9 | NC_002577.1 | + | 128872 | 1.11 | 0.00234 |
|
Target: 5'- uUCCUCCUUCGCGGGGUGCUUGAGAUCa -3' miRNA: 3'- -AGGAGGAAGCGCCCCACGAACUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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