Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9590 | 3' | -55.7 | NC_002577.1 | + | 130398 | 0.72 | 0.62915 |
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Target: 5'- aCUCCaUCCUUcGGUGcGGUGCCcGAGAa -3' miRNA: 3'- -GAGGcAGGAA-UCGCaCCACGGaCUCU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 3215 | 0.72 | 0.62915 |
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Target: 5'- aCUCCaUCCUUcGGUGcGGUGCCcGAGAa -3' miRNA: 3'- -GAGGcAGGAA-UCGCaCCACGGaCUCU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 130634 | 0.75 | 0.479968 |
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Target: 5'- uCUCCGUCCaucGaCGUGGUGCUUGAuGAc -3' miRNA: 3'- -GAGGCAGGaauC-GCACCACGGACU-CU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 4748 | 0.76 | 0.407581 |
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Target: 5'- gCUCCGcuuccUCCUUcGCGgGGUGCUUGAGAu -3' miRNA: 3'- -GAGGC-----AGGAAuCGCaCCACGGACUCU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 129102 | 0.76 | 0.407581 |
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Target: 5'- uCUCCGUCCaucGGCGUGGUGCUcGAu- -3' miRNA: 3'- -GAGGCAGGaa-UCGCACCACGGaCUcu -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 4512 | 0.76 | 0.407581 |
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Target: 5'- uCUCCGUCCaucGGCGUGGUGCUcGAu- -3' miRNA: 3'- -GAGGCAGGaa-UCGCACCACGGaCUcu -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 128865 | 0.76 | 0.407581 |
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Target: 5'- gCUCCGcuuccUCCUUcGCGgGGUGCUUGAGAu -3' miRNA: 3'- -GAGGC-----AGGAAuCGCaCCACGGACUCU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 128734 | 1 | 0.012567 |
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Target: 5'- gCUCCGUCC-UAGCGUGGUGCCUGAGAu -3' miRNA: 3'- -GAGGCAGGaAUCGCACCACGGACUCU- -5' |
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| 9590 | 3' | -55.7 | NC_002577.1 | + | 4880 | 1.09 | 0.00342 |
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Target: 5'- gCUCCGUCCUUAGCGUGGUGCCUGAGAu -3' miRNA: 3'- -GAGGCAGGAAUCGCACCACGGACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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