Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9593 | 5' | -45.4 | NC_002577.1 | + | 94360 | 0.66 | 0.99999 |
Target: 5'- cCGuUUUGGUGUGaaagaggUUagcacagauaucgUCCgCUUCCAUCa -3' miRNA: 3'- -GC-AAACCACACaa-----AA-------------AGG-GAAGGUAG- -5' |
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9593 | 5' | -45.4 | NC_002577.1 | + | 126944 | 0.73 | 0.988196 |
Target: 5'- aGUUUuccGGUuUGccUUUCCCUUCCAUCg -3' miRNA: 3'- gCAAA---CCAcACaaAAAGGGAAGGUAG- -5' |
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9593 | 5' | -45.4 | NC_002577.1 | + | 6670 | 0.73 | 0.988196 |
Target: 5'- aGUUUuccGGUuUGccUUUCCCUUCCAUCg -3' miRNA: 3'- gCAAA---CCAcACaaAAAGGGAAGGUAG- -5' |
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9593 | 5' | -45.4 | NC_002577.1 | + | 6827 | 1.13 | 0.020155 |
Target: 5'- cCGUUUGGUGUGUUUUUCCCUUCCAUCg -3' miRNA: 3'- -GCAAACCACACAAAAAGGGAAGGUAG- -5' |
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9593 | 5' | -45.4 | NC_002577.1 | + | 126786 | 1.13 | 0.020155 |
Target: 5'- cCGUUUGGUGUGUUUUUCCCUUCCAUCg -3' miRNA: 3'- -GCAAACCACACAAAAAGGGAAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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