miRNA: 3'- -UG-GGGGUG-UGu--G-GGGGUGUGUGUGGc -5'
miRNA: 3'- -UGGGGGuGUGUGGGGgUGUGUGUg-GC- -5'
miRNA: 3'- ugGGGGUGUGUGGGGgUGUGUGUGGc -5'
miRNA: 3'- -UGGGGGuGUGuGGGGGu---------GUG-UGUGGC- -5'
miRNA: 3'- -UGGGGGUGUGUGGGGGUGUGUGUGGC- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9594 | 3' | -60.6 | NC_002577.1 | + | 139118 | 0.7 | 0.501937 |
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Target: 5'- gACgCCCCACgGCucuCgUCCCGCACGCGCCu -3' miRNA: 3'- -UG-GGGGUG-UGu--G-GGGGUGUGUGUGGc -5' |
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| 9594 | 3' | -60.6 | NC_002577.1 | + | 159918 | 0.71 | 0.45645 |
|
Target: 5'- gACCCCCuCGCACUCCaGCGCGCAagCGu -3' miRNA: 3'- -UGGGGGuGUGUGGGGgUGUGUGUg-GC- -5' |
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| 9594 | 3' | -60.6 | NC_002577.1 | + | 139755 | 0.71 | 0.447618 |
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Target: 5'- -gCUCCGCGCACCCCgACGUACGCUu -3' miRNA: 3'- ugGGGGUGUGUGGGGgUGUGUGUGGc -5' |
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| 9594 | 3' | -60.6 | NC_002577.1 | + | 136916 | 0.71 | 0.438882 |
|
Target: 5'- gGCCCCC-CACcCCCCCugaagcgugcCGCgACGCCGg -3' miRNA: 3'- -UGGGGGuGUGuGGGGGu---------GUG-UGUGGC- -5' |
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| 9594 | 3' | -60.6 | NC_002577.1 | + | 8640 | 1.07 | 0.001723 |
|
Target: 5'- cACCCCCACACACCCCCACACACACCGc -3' miRNA: 3'- -UGGGGGUGUGUGGGGGUGUGUGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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