Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9594 | 5' | -52.6 | NC_002577.1 | + | 84714 | 0.66 | 0.985193 |
Target: 5'- uGGCGuuuCUGcgCGUAGUuuuuGCGUAUGGGUu -3' miRNA: 3'- -UCGCc--GACa-GCAUCA----UGCAUAUCCGc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 37541 | 0.66 | 0.985193 |
Target: 5'- uGGCGGCUG-CGUGcGUugGacUAGGg- -3' miRNA: 3'- -UCGCCGACaGCAU-CAugCauAUCCgc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 103306 | 0.66 | 0.983346 |
Target: 5'- cAGCGag-GUCGUGGUACaUGgcGGCGa -3' miRNA: 3'- -UCGCcgaCAGCAUCAUGcAUauCCGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 31631 | 0.66 | 0.978905 |
Target: 5'- uAGUGGCgcgGUaCGUgcacaugauuuugGGUGCGUAcaUAGGCc -3' miRNA: 3'- -UCGCCGa--CA-GCA-------------UCAUGCAU--AUCCGc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 59282 | 0.66 | 0.976755 |
Target: 5'- gGGCGGCUGUagcacauauuaGUAG-ACGU---GGCGa -3' miRNA: 3'- -UCGCCGACAg----------CAUCaUGCAuauCCGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 92165 | 0.67 | 0.973643 |
Target: 5'- uGCGGCUGUCGgcaucaacgcUACGaacUGGGCa -3' miRNA: 3'- uCGCCGACAGCauc-------AUGCau-AUCCGc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 104545 | 0.68 | 0.95429 |
Target: 5'- cGGCGGCUGUUGgaacccGUGCcucgaGUcgAGGCu -3' miRNA: 3'- -UCGCCGACAGCau----CAUG-----CAuaUCCGc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 99586 | 0.69 | 0.92974 |
Target: 5'- uAGCGGCUGUUGUGGgacuagcuuugauuUugGgaAUGGGUa -3' miRNA: 3'- -UCGCCGACAGCAUC--------------AugCa-UAUCCGc -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 141730 | 0.71 | 0.851448 |
Target: 5'- cGUGGCUGUgGU-GUGUGUAgGGGCGg -3' miRNA: 3'- uCGCCGACAgCAuCAUGCAUaUCCGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 155608 | 0.71 | 0.851448 |
Target: 5'- cGUGGCUGUgGU-GUGUGUAgGGGCGg -3' miRNA: 3'- uCGCCGACAgCAuCAUGCAUaUCCGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 9247 | 0.74 | 0.681945 |
Target: 5'- gAGCGGuCUcGUCGUAGUGCGUAUGaacGUGu -3' miRNA: 3'- -UCGCC-GA-CAGCAUCAUGCAUAUc--CGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 124367 | 0.74 | 0.681945 |
Target: 5'- gAGCGGuCUcGUCGUAGUGCGUAUGaacGUGu -3' miRNA: 3'- -UCGCC-GA-CAGCAUCAUGCAUAUc--CGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 103139 | 0.78 | 0.497512 |
Target: 5'- gAGCGGCgUGcagaUCGUGGUGacgcgcCGUAUGGGCGg -3' miRNA: 3'- -UCGCCG-AC----AGCAUCAU------GCAUAUCCGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 8605 | 1.03 | 0.016729 |
Target: 5'- gAGCGGCUGUCGUAGUACGUAUAGcGCGa -3' miRNA: 3'- -UCGCCGACAGCAUCAUGCAUAUC-CGC- -5' |
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9594 | 5' | -52.6 | NC_002577.1 | + | 125008 | 1.09 | 0.00699 |
Target: 5'- gAGCGGCUGUCGUAGUACGUAUAGGCGc -3' miRNA: 3'- -UCGCCGACAGCAUCAUGCAUAUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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