Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9597 | 5' | -52.7 | NC_002577.1 | + | 142907 | 0.66 | 0.978766 |
Target: 5'- uGGGUcAGGGGGcgCUG-UUUGUUGUg -3' miRNA: 3'- uCCCA-UCUCCCaaGGCgAAACAACGu -5' |
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9597 | 5' | -52.7 | NC_002577.1 | + | 156106 | 0.68 | 0.94326 |
Target: 5'- cGGGGUGGcGGGUggcugcggagaaaaUCCGCcgUUGUcgGCu -3' miRNA: 3'- -UCCCAUCuCCCA--------------AGGCGa-AACAa-CGu -5' |
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9597 | 5' | -52.7 | NC_002577.1 | + | 141232 | 0.68 | 0.94326 |
Target: 5'- cGGGGUGGcGGGUggcugcggagaaaaUCCGCcgUUGUcgGCu -3' miRNA: 3'- -UCCCAUCuCCCA--------------AGGCGa-AACAa-CGu -5' |
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9597 | 5' | -52.7 | NC_002577.1 | + | 118344 | 0.72 | 0.76702 |
Target: 5'- uGGGaAGAGGGUUCCccccacuacgucGCUUUGagGCGu -3' miRNA: 3'- uCCCaUCUCCCAAGG------------CGAAACaaCGU- -5' |
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9597 | 5' | -52.7 | NC_002577.1 | + | 9966 | 0.96 | 0.041129 |
Target: 5'- cAGGGUAGAGGGUcUCGCUUUGUUGCAc -3' miRNA: 3'- -UCCCAUCUCCCAaGGCGAAACAACGU- -5' |
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9597 | 5' | -52.7 | NC_002577.1 | + | 123648 | 0.96 | 0.041129 |
Target: 5'- cAGGGUAGAGGGUcUCGCUUUGUUGCAc -3' miRNA: 3'- -UCCCAUCUCCCAaGGCGAAACAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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