Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9598 | 3' | -53.8 | NC_002577.1 | + | 154278 | 0.66 | 0.955491 |
Target: 5'- uGCgaUGUGGCUUcGCCGCAAAGuccuuucccauacuAGGCg -3' miRNA: 3'- uCGa-ACGCCGGA-CGGCGUUUU--------------UCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 139565 | 0.66 | 0.952657 |
Target: 5'- cGGCUcGUGGUCUGCCGUgcuuuccGGCg -3' miRNA: 3'- -UCGAaCGCCGGACGGCGuuuuu--CUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 142959 | 0.66 | 0.952657 |
Target: 5'- gGGCgUGCGGCgaGCacccagCGCGGAAcGACUc -3' miRNA: 3'- -UCGaACGCCGgaCG------GCGUUUUuCUGA- -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 157772 | 0.66 | 0.952657 |
Target: 5'- cGGCUcGUGGUCUGCCGUgcuuuccGGCg -3' miRNA: 3'- -UCGAaCGCCGGACGGCGuuuuu--CUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 4422 | 0.66 | 0.939151 |
Target: 5'- uGGCUUGCuuguaGGCUguccGUCGgAGGAAGACa -3' miRNA: 3'- -UCGAACG-----CCGGa---CGGCgUUUUUCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 129192 | 0.66 | 0.939151 |
Target: 5'- uGGCUUGCuuguaGGCUguccGUCGgAGGAAGACa -3' miRNA: 3'- -UCGAACG-----CCGGa---CGGCgUUUUUCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 90831 | 0.67 | 0.933636 |
Target: 5'- uGCUgccgccGCGGCCaGCCgaacaaaaaacugGCAAAGAGAUUc -3' miRNA: 3'- uCGAa-----CGCCGGaCGG-------------CGUUUUUCUGA- -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 54824 | 0.67 | 0.927816 |
Target: 5'- gAGCUgcuucUGCGGCUgaacuugccgacGCCGCAGccgauGAGGCUc -3' miRNA: 3'- -UCGA-----ACGCCGGa-----------CGGCGUUu----UUCUGA- -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 112777 | 0.69 | 0.854797 |
Target: 5'- cAGCUUGCGGCUU-CCuuuuuuugGCAAGAuAGACg -3' miRNA: 3'- -UCGAACGCCGGAcGG--------CGUUUU-UCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 81278 | 0.69 | 0.838341 |
Target: 5'- cGCgcaGCGGCgaCUGCUGCAGAAAG-Cg -3' miRNA: 3'- uCGaa-CGCCG--GACGGCGUUUUUCuGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 111745 | 0.7 | 0.79388 |
Target: 5'- aGGCcaccCGGCCUGCCGCuAGAAcauccaGGACUc -3' miRNA: 3'- -UCGaac-GCCGGACGGCG-UUUU------UCUGA- -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 18103 | 0.71 | 0.77495 |
Target: 5'- cGUUUGCGuCCUGCaCGCAcuuuAAAGACg -3' miRNA: 3'- uCGAACGCcGGACG-GCGUu---UUUCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 138488 | 0.74 | 0.558541 |
Target: 5'- aGGCc-GCGGCCgcgGCCGC-GGAGGACg -3' miRNA: 3'- -UCGaaCGCCGGa--CGGCGuUUUUCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 158849 | 0.74 | 0.558541 |
Target: 5'- aGGCc-GCGGCCgcgGCCGC-GGAGGACg -3' miRNA: 3'- -UCGaaCGCCGGa--CGGCGuUUUUCUGa -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 10411 | 1.07 | 0.005145 |
Target: 5'- aAGCUUGCGGCCUGCCGCAAAAAGACUc -3' miRNA: 3'- -UCGAACGCCGGACGGCGUUUUUCUGA- -5' |
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9598 | 3' | -53.8 | NC_002577.1 | + | 123203 | 1.07 | 0.005145 |
Target: 5'- aAGCUUGCGGCCUGCCGCAAAAAGACUc -3' miRNA: 3'- -UCGAACGCCGGACGGCGUUUUUCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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