Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9598 | 5' | -54.9 | NC_002577.1 | + | 138272 | 0.71 | 0.72629 |
Target: 5'- gAUGGCgcccgccggCCGCGGccGCGCCGUCCUc -3' miRNA: 3'- -UAUCGaaua-----GGCGCUa-UGCGGCGGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 159066 | 0.71 | 0.72629 |
Target: 5'- gAUGGCgcccgccggCCGCGGccGCGCCGUCCUc -3' miRNA: 3'- -UAUCGaaua-----GGCGCUa-UGCGGCGGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 43161 | 0.73 | 0.614016 |
Target: 5'- -gAGCUUGUCCGCGuaagagaauuuuUGCGaaGCCCa -3' miRNA: 3'- uaUCGAAUAGGCGCu-----------AUGCggCGGGg -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 123238 | 1.09 | 0.003719 |
Target: 5'- aAUAGCUUAUCCGCGAUACGCCGCCCCc -3' miRNA: 3'- -UAUCGAAUAGGCGCUAUGCGGCGGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 10376 | 1.09 | 0.003719 |
Target: 5'- aAUAGCUUAUCCGCGAUACGCCGCCCCc -3' miRNA: 3'- -UAUCGAAUAGGCGCUAUGCGGCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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