Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9599 | 5' | -54.9 | NC_002577.1 | + | 17581 | 0.66 | 0.939973 |
Target: 5'- -gCCCagUgGCGCGAaacGGGCgGAGGUCCGAu -3' miRNA: 3'- uaGGG--AgCGUGCU---CUUG-CUCUAGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 139511 | 0.67 | 0.928529 |
Target: 5'- cUCCCUUGgACGAGGACuGGAacggaacgccgcccUCUGAa -3' miRNA: 3'- uAGGGAGCgUGCUCUUGcUCU--------------AGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 157826 | 0.67 | 0.928529 |
Target: 5'- cUCCCUUGgACGAGGACuGGAacggaacgccgcccUCUGAa -3' miRNA: 3'- uAGGGAGCgUGCUCUUGcUCU--------------AGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 158597 | 0.67 | 0.919256 |
Target: 5'- cGUCCCUCGCGCcGGAcggcugACgGAGGugUCUGAa -3' miRNA: 3'- -UAGGGAGCGUGcUCU------UG-CUCU--AGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 138740 | 0.67 | 0.919256 |
Target: 5'- cGUCCCUCGCGCcGGAcggcugACgGAGGugUCUGAa -3' miRNA: 3'- -UAGGGAGCGUGcUCU------UG-CUCU--AGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 123098 | 0.67 | 0.913479 |
Target: 5'- --aCCUCGcCGCGGGuGCGGGG-CCGGg -3' miRNA: 3'- uagGGAGC-GUGCUCuUGCUCUaGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 10516 | 0.67 | 0.913479 |
Target: 5'- --aCCUCGcCGCGGGuGCGGGG-CCGGg -3' miRNA: 3'- uagGGAGC-GUGCUCuUGCUCUaGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 114450 | 0.7 | 0.809346 |
Target: 5'- -aCCCUCGUGCGAuauGAACGAGcgcUCCa- -3' miRNA: 3'- uaGGGAGCGUGCU---CUUGCUCu--AGGcu -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 11219 | 0.72 | 0.684616 |
Target: 5'- -cCCCUCcCACGGGGGCG-GAUUCGGg -3' miRNA: 3'- uaGGGAGcGUGCUCUUGCuCUAGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 122395 | 0.72 | 0.684616 |
Target: 5'- -cCCCUCcCACGGGGGCG-GAUUCGGg -3' miRNA: 3'- uaGGGAGcGUGCUCUUGCuCUAGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 10798 | 1.07 | 0.005639 |
Target: 5'- gAUCCCUCGCACGAGAACGAGAUCCGAc -3' miRNA: 3'- -UAGGGAGCGUGCUCUUGCUCUAGGCU- -5' |
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9599 | 5' | -54.9 | NC_002577.1 | + | 122815 | 1.07 | 0.005639 |
Target: 5'- gAUCCCUCGCACGAGAACGAGAUCCGAc -3' miRNA: 3'- -UAGGGAGCGUGCUCUUGCUCUAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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