Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 96 | 3' | -60.7 | AC_000006.1 | + | 26149 | 0.96 | 0.001312 |
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Target: 5'- cAUCAGCGaGAUGCCCCUGCGCCGCUGg -3' miRNA: 3'- -UAGUCGCcUUACGGGGACGCGGCGAC- -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 14103 | 0.69 | 0.16105 |
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Target: 5'- -gCGGCGGAGUcggGCCCCUGCaCgGuCUGg -3' miRNA: 3'- uaGUCGCCUUA---CGGGGACGcGgC-GAC- -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 24521 | 0.69 | 0.184899 |
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Target: 5'- aAUCGGCGGggUgaccaaGUCCCgcuCGCCGCg- -3' miRNA: 3'- -UAGUCGCCuuA------CGGGGac-GCGGCGac -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 10956 | 0.68 | 0.206173 |
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Target: 5'- -gCAGaCGGGGaucaGCCCC-GCGCCGCg- -3' miRNA: 3'- uaGUC-GCCUUa---CGGGGaCGCGGCGac -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 10710 | 0.68 | 0.206173 |
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Target: 5'- -gCAGCGGcAGUggcacucuagcaGCCCCU-CGCCGCUc -3' miRNA: 3'- uaGUCGCC-UUA------------CGGGGAcGCGGCGAc -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 2282 | 0.68 | 0.211813 |
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Target: 5'- uGUCgGGCGGGAgGCaCCCUGCugGCCGUg- -3' miRNA: 3'- -UAG-UCGCCUUaCG-GGGACG--CGGCGac -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 16097 | 0.67 | 0.248487 |
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Target: 5'- -gCAGCaGGugcUGCCCCaggcgGUGCUGCUGc -3' miRNA: 3'- uaGUCG-CCuu-ACGGGGa----CGCGGCGAC- -5' |
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| 96 | 3' | -60.7 | AC_000006.1 | + | 28778 | 0.66 | 0.290257 |
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Target: 5'- cUCuGCuGGAcuaacgaGCCCCUGCGCgGCg- -3' miRNA: 3'- uAGuCG-CCUua-----CGGGGACGCGgCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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