Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
960 | 3' | -58.1 | NC_000902.1 | + | 14167 | 0.66 | 0.636602 |
Target: 5'- aCCCGAauauuuuuuaccuucCGGCgacaaaugACGCCGAUGAC-GGCu -3' miRNA: 3'- aGGGCU---------------GCCG--------UGUGGUUGCUGaCCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 34340 | 0.66 | 0.621692 |
Target: 5'- gUCCUGuACGuCACGCCGcgucaguggaAUGACUGGUa -3' miRNA: 3'- -AGGGC-UGCcGUGUGGU----------UGCUGACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 5782 | 0.66 | 0.621692 |
Target: 5'- cCCUGAaaGCACA---GCGGCUGGCu -3' miRNA: 3'- aGGGCUgcCGUGUgguUGCUGACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 42567 | 0.66 | 0.621692 |
Target: 5'- uUUCCGuucugaauaACGGCACuCCGGauaACUGGCGa -3' miRNA: 3'- -AGGGC---------UGCCGUGuGGUUgc-UGACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 25693 | 0.66 | 0.620628 |
Target: 5'- aCCCGcaccaucACGGUAUGCUGugGugUGGUu -3' miRNA: 3'- aGGGC-------UGCCGUGUGGUugCugACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 50884 | 0.66 | 0.611051 |
Target: 5'- uUCCCGAaacCGG-ACACgCAAa-ACUGGCGa -3' miRNA: 3'- -AGGGCU---GCCgUGUG-GUUgcUGACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 29263 | 0.66 | 0.600427 |
Target: 5'- -gCUGACGGCACAggauuauaaauuCCAuuuuuACGcCUGGUGg -3' miRNA: 3'- agGGCUGCCGUGU------------GGU-----UGCuGACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 25974 | 0.66 | 0.600427 |
Target: 5'- aUCCCGuCGugaccaggagaGCGCGCUGGCGuGCUGGgGa -3' miRNA: 3'- -AGGGCuGC-----------CGUGUGGUUGC-UGACCgC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 3709 | 0.67 | 0.558262 |
Target: 5'- -gCUGGCugugcaaaagaGGCGCGCaagGAUGGCUGGCGa -3' miRNA: 3'- agGGCUG-----------CCGUGUGg--UUGCUGACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 18053 | 0.68 | 0.506841 |
Target: 5'- gCCUGACGG---GCCGACGAaccuuCUGGCa -3' miRNA: 3'- aGGGCUGCCgugUGGUUGCU-----GACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 30870 | 0.68 | 0.483862 |
Target: 5'- uUCCCGGgaaUGGCucagguuaucgauuAUGCCAuugAUGACUGGCGu -3' miRNA: 3'- -AGGGCU---GCCG--------------UGUGGU---UGCUGACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 23245 | 0.69 | 0.457544 |
Target: 5'- -gCCGAUGGCACAUaCAgugacGCGAgUGGUGc -3' miRNA: 3'- agGGCUGCCGUGUG-GU-----UGCUgACCGC- -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 6212 | 0.69 | 0.410932 |
Target: 5'- cUCCCGAUGGUuuauGCAgUGACGGCaaUGGCc -3' miRNA: 3'- -AGGGCUGCCG----UGUgGUUGCUG--ACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 27048 | 0.75 | 0.189039 |
Target: 5'- gUCCUGGCGGUugGCUAugGugcgcugugGCUGGCa -3' miRNA: 3'- -AGGGCUGCCGugUGGUugC---------UGACCGc -5' |
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960 | 3' | -58.1 | NC_000902.1 | + | 35952 | 1.09 | 0.000622 |
Target: 5'- cUCCCGACGGCACACCAACGACUGGCGg -3' miRNA: 3'- -AGGGCUGCCGUGUGGUUGCUGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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