Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9601 | 3' | -47.4 | NC_002577.1 | + | 59434 | 0.66 | 0.999674 |
Target: 5'- uUUCGUAUgGCGAgaACCAGgggAGGAUa -3' miRNA: 3'- cAAGCGUGgCGCU--UGGUUauaUUCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 153378 | 0.67 | 0.999482 |
Target: 5'- --gCGUACCGCGcGCCAauuuucGUGUAGGu- -3' miRNA: 3'- caaGCGUGGCGCuUGGU------UAUAUUCua -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 7894 | 0.67 | 0.999145 |
Target: 5'- -aUCGCGagcgcgauccgaauCCGCGGGCCGAg--GGGAa -3' miRNA: 3'- caAGCGU--------------GGCGCUUGGUUauaUUCUa -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 124765 | 0.67 | 0.998927 |
Target: 5'- --gCGCACCGCGGGCCucggcgcGGUa -3' miRNA: 3'- caaGCGUGGCGCUUGGuuauauuCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 8849 | 0.67 | 0.998927 |
Target: 5'- --gCGCACCGCGGGCCucggcgcGGUa -3' miRNA: 3'- caaGCGUGGCGCUUGGuuauauuCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 70742 | 0.67 | 0.998789 |
Target: 5'- uGUUCGgACCGCaugGCCAAUuugugauacacgGUGAGAUg -3' miRNA: 3'- -CAAGCgUGGCGcu-UGGUUA------------UAUUCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 61175 | 0.67 | 0.998764 |
Target: 5'- uUUCGUACCGCGGcgcgccuuuccacACUAucgAUGAGAc -3' miRNA: 3'- cAAGCGUGGCGCU-------------UGGUua-UAUUCUa -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 58182 | 0.69 | 0.996366 |
Target: 5'- -aUCGCGCCGaCGAugCAGgcgcGGAUa -3' miRNA: 3'- caAGCGUGGC-GCUugGUUauauUCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 89298 | 0.69 | 0.995715 |
Target: 5'- -aUCGCACagCGCGAaaucGCCAGUAUAAc-- -3' miRNA: 3'- caAGCGUG--GCGCU----UGGUUAUAUUcua -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 125696 | 0.7 | 0.993171 |
Target: 5'- -aUCGCgAUCGCGAACCGGUAa----- -3' miRNA: 3'- caAGCG-UGGCGCUUGGUUAUauucua -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 7918 | 0.7 | 0.993171 |
Target: 5'- -aUCGCgAUCGCGAACCGGUAa----- -3' miRNA: 3'- caAGCG-UGGCGCUUGGUUAUauucua -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 46981 | 0.72 | 0.975335 |
Target: 5'- --aCGgGCCGCGu-CCGAUGUAAGGa -3' miRNA: 3'- caaGCgUGGCGCuuGGUUAUAUUCUa -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 61158 | 0.74 | 0.941103 |
Target: 5'- --cCGCACCGaGGACCGuUAUGAGAg -3' miRNA: 3'- caaGCGUGGCgCUUGGUuAUAUUCUa -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 11368 | 1.06 | 0.027638 |
Target: 5'- cGUUCGCACCGCGAACCAAUAUAAGAUu -3' miRNA: 3'- -CAAGCGUGGCGCUUGGUUAUAUUCUA- -5' |
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9601 | 3' | -47.4 | NC_002577.1 | + | 122246 | 1.06 | 0.027638 |
Target: 5'- cGUUCGCACCGCGAACCAAUAUAAGAUu -3' miRNA: 3'- -CAAGCGUGGCGCUUGGUUAUAUUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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