Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9601 | 5' | -44.4 | NC_002577.1 | + | 35622 | 0.66 | 0.999997 |
Target: 5'- ---cAUAgaAUUGGaCGCAGaGUGCGAg -3' miRNA: 3'- auuaUAUaaUAACC-GCGUUcCACGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 153723 | 0.67 | 0.999994 |
Target: 5'- gAGUGUGggugggUGGCG-AAGGUGCuGAg -3' miRNA: 3'- aUUAUAUaaua--ACCGCgUUCCACG-CU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 75367 | 0.67 | 0.99999 |
Target: 5'- cGAUGUAg---UGGCGCcgaugaugacucuguGGUGCGGu -3' miRNA: 3'- aUUAUAUaauaACCGCGuu-------------CCACGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 140288 | 0.69 | 0.999849 |
Target: 5'- --cUAUGUUGUgGcGCGCGGGGgcucGCGAc -3' miRNA: 3'- auuAUAUAAUAaC-CGCGUUCCa---CGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 157050 | 0.69 | 0.999849 |
Target: 5'- --cUAUGUUGUgGcGCGCGGGGgcucGCGAc -3' miRNA: 3'- auuAUAUAAUAaC-CGCGUUCCa---CGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 160788 | 0.7 | 0.999673 |
Target: 5'- -----------aGGCGuCGAGGUGCGGg -3' miRNA: 3'- auuauauaauaaCCGC-GUUCCACGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 136550 | 0.7 | 0.999673 |
Target: 5'- -----------aGGCGuCGAGGUGCGGg -3' miRNA: 3'- auuauauaauaaCCGC-GUUCCACGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 11334 | 1.1 | 0.033752 |
Target: 5'- aUAAUAUAUUAUUGGCGCAAGGUGCGAa -3' miRNA: 3'- -AUUAUAUAAUAACCGCGUUCCACGCU- -5' |
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9601 | 5' | -44.4 | NC_002577.1 | + | 122280 | 1.1 | 0.033752 |
Target: 5'- aUAAUAUAUUAUUGGCGCAAGGUGCGAa -3' miRNA: 3'- -AUUAUAUAAUAACCGCGUUCCACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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