Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9603 | 5' | -53.5 | NC_002577.1 | + | 100996 | 0.66 | 0.970523 |
Target: 5'- gCGCggcCGCUGGCAUAuggcgguacagguCCUCGggcagUCGACg -3' miRNA: 3'- -GCGa--GCGACUGUAUu------------GGAGCg----AGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 136613 | 0.66 | 0.968729 |
Target: 5'- uCGCUCGCgUGGCG-AGCaCUCcCUCGcACg -3' miRNA: 3'- -GCGAGCG-ACUGUaUUG-GAGcGAGC-UG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 160724 | 0.66 | 0.968729 |
Target: 5'- uCGCUCGCgUGGCG-AGCaCUCcCUCGcACg -3' miRNA: 3'- -GCGAGCG-ACUGUaUUG-GAGcGAGC-UG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 144387 | 0.67 | 0.958593 |
Target: 5'- uGCUCuCaGAag-AGCCUUGCUCGAUa -3' miRNA: 3'- gCGAGcGaCUguaUUGGAGCGAGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 136028 | 0.68 | 0.932064 |
Target: 5'- aGCUaCGacgGuCAUAACCUCGCggGACg -3' miRNA: 3'- gCGA-GCga-CuGUAUUGGAGCGagCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 161310 | 0.68 | 0.932064 |
Target: 5'- aGCUaCGacgGuCAUAACCUCGCggGACg -3' miRNA: 3'- gCGA-GCga-CuGUAUUGGAGCGagCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 86610 | 0.68 | 0.932064 |
Target: 5'- uGCUCGaUUGGCAgucccGGCCcCGCUCuGGCg -3' miRNA: 3'- gCGAGC-GACUGUa----UUGGaGCGAG-CUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 11554 | 0.68 | 0.909522 |
Target: 5'- uCGCUC-CcGACGgGGCuCUCGUUCGGCg -3' miRNA: 3'- -GCGAGcGaCUGUaUUG-GAGCGAGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 122060 | 0.68 | 0.909522 |
Target: 5'- uCGCUC-CcGACGgGGCuCUCGUUCGGCg -3' miRNA: 3'- -GCGAGcGaCUGUaUUG-GAGCGAGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 154743 | 0.69 | 0.903279 |
Target: 5'- aGUUCGUUGGCGUGGCCacgaGCUCu-- -3' miRNA: 3'- gCGAGCGACUGUAUUGGag--CGAGcug -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 34856 | 0.69 | 0.896796 |
Target: 5'- aCGCUauugagCGCUaGAguUGACCUUGCcCGACa -3' miRNA: 3'- -GCGA------GCGA-CUguAUUGGAGCGaGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 39476 | 0.69 | 0.896796 |
Target: 5'- gCGCUCGCcugGugAUAGCCUCgGCUg--- -3' miRNA: 3'- -GCGAGCGa--CugUAUUGGAG-CGAgcug -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 125654 | 0.7 | 0.853088 |
Target: 5'- gCGCgggCGCgGGCAgggaguUAACCUCGCgaucCGACc -3' miRNA: 3'- -GCGa--GCGaCUGU------AUUGGAGCGa---GCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 52600 | 0.73 | 0.725536 |
Target: 5'- --gUCGCUGACAUcAAgC-CGCUCGACa -3' miRNA: 3'- gcgAGCGACUGUA-UUgGaGCGAGCUG- -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 142867 | 0.73 | 0.684996 |
Target: 5'- cCGCccuUCGUUGACGUgucggcGACCUCuGCUCGAg -3' miRNA: 3'- -GCG---AGCGACUGUA------UUGGAG-CGAGCUg -5' |
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9603 | 5' | -53.5 | NC_002577.1 | + | 19287 | 1.09 | 0.005163 |
Target: 5'- cCGCUCGCUGACAUAACCUCGCUCGACc -3' miRNA: 3'- -GCGAGCGACUGUAUUGGAGCGAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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