Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9604 | 3' | -52.9 | NC_002577.1 | + | 108545 | 0.66 | 0.980505 |
Target: 5'- gGCUgguuaUACgUCUgGCGCUcgugcaguugGC-CUAUCCGGa -3' miRNA: 3'- -CGA-----AUGgAGA-CGCGA----------CGaGAUAGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 60137 | 0.66 | 0.978248 |
Target: 5'- uGCUcGCCuUCUGaCGCuUGCUCagAUCCa- -3' miRNA: 3'- -CGAaUGG-AGAC-GCG-ACGAGa-UAGGcc -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 105368 | 0.67 | 0.967265 |
Target: 5'- ----uUCUCUGuCGCUGCUCUugaCCGa -3' miRNA: 3'- cgaauGGAGAC-GCGACGAGAua-GGCc -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 154412 | 0.68 | 0.929505 |
Target: 5'- uGCUUACCUC-GUGaUUGCUgUcgUCGGg -3' miRNA: 3'- -CGAAUGGAGaCGC-GACGAgAuaGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 27787 | 0.69 | 0.924104 |
Target: 5'- uGCgaACCUCUGCcggGCgaucgaugUGCUCcccGUCCGGg -3' miRNA: 3'- -CGaaUGGAGACG---CG--------ACGAGa--UAGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 70807 | 0.69 | 0.906429 |
Target: 5'- cGCgcagUACCUCcgGCGUUGCggcagCUAugacauaggaaaUCCGGu -3' miRNA: 3'- -CGa---AUGGAGa-CGCGACGa----GAU------------AGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 22388 | 0.69 | 0.906429 |
Target: 5'- aGCUUGCaauaaauaUUCUGCGCUGCgaccuuugUCgugCCGGu -3' miRNA: 3'- -CGAAUG--------GAGACGCGACG--------AGauaGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 112107 | 0.7 | 0.893435 |
Target: 5'- uGUUUGCCUUUG-GacaUGC-CUAUCCGGu -3' miRNA: 3'- -CGAAUGGAGACgCg--ACGaGAUAGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 147445 | 0.7 | 0.89276 |
Target: 5'- cGCUgAUCUCUgccGCGCUGCUCUggaauacAUCCc- -3' miRNA: 3'- -CGAaUGGAGA---CGCGACGAGA-------UAGGcc -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 23581 | 0.7 | 0.872189 |
Target: 5'- aGCgcgAUCUCUGCaGUUGCU--AUCCGGc -3' miRNA: 3'- -CGaa-UGGAGACG-CGACGAgaUAGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 20992 | 0.76 | 0.57579 |
Target: 5'- ----cCCUCUGCuCcGCUCUAUCCGGa -3' miRNA: 3'- cgaauGGAGACGcGaCGAGAUAGGCC- -5' |
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9604 | 3' | -52.9 | NC_002577.1 | + | 21215 | 1.14 | 0.003112 |
Target: 5'- uGCUUACCUCUGCGCUGCUCUAUCCGGg -3' miRNA: 3'- -CGAAUGGAGACGCGACGAGAUAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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