Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9605 | 3' | -50.8 | NC_002577.1 | + | 33766 | 0.66 | 0.993508 |
Target: 5'- -gGCGGAAgacaaGCUCCuguaugaaUCGGGCGCa---- -3' miRNA: 3'- caUGCUUU-----UGAGG--------AGCCCGCGauuca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 33448 | 0.67 | 0.987234 |
Target: 5'- -cGCGAAc-CUUCUCGGGUGCUc--- -3' miRNA: 3'- caUGCUUuuGAGGAGCCCGCGAuuca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 59817 | 0.68 | 0.981035 |
Target: 5'- -gGCGAGAGCUCCguaaugCGccgaaggaaaaacuGGCGCUGAa- -3' miRNA: 3'- caUGCUUUUGAGGa-----GC--------------CCGCGAUUca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 61999 | 0.68 | 0.979471 |
Target: 5'- uUACuAAAAUcgUCCUCGGGCGCg---- -3' miRNA: 3'- cAUGcUUUUG--AGGAGCCCGCGauuca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 138004 | 0.68 | 0.971673 |
Target: 5'- -aGCGAAAuCUgCUUUGGGCGCUAc-- -3' miRNA: 3'- caUGCUUUuGA-GGAGCCCGCGAUuca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 159334 | 0.68 | 0.971673 |
Target: 5'- -aGCGAAAuCUgCUUUGGGCGCUAc-- -3' miRNA: 3'- caUGCUUUuGA-GGAGCCCGCGAUuca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 54197 | 0.69 | 0.965412 |
Target: 5'- -gAUGAAAauacGCUCCgaaccuaCGGGCGCUAAa- -3' miRNA: 3'- caUGCUUU----UGAGGa------GCCCGCGAUUca -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 150656 | 0.7 | 0.936033 |
Target: 5'- -gGCGGGAAUacacuuUCCUggaCGGGCGUUGAGg -3' miRNA: 3'- caUGCUUUUG------AGGA---GCCCGCGAUUCa -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 154527 | 0.7 | 0.936033 |
Target: 5'- -cACGG--GCcCCUUGGGUGCUGGGg -3' miRNA: 3'- caUGCUuuUGaGGAGCCCGCGAUUCa -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 137403 | 0.73 | 0.848924 |
Target: 5'- -cGCGAAcgguucGGCUCCUCGGGgGCgccagGAGc -3' miRNA: 3'- caUGCUU------UUGAGGAGCCCgCGa----UUCa -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 159934 | 0.73 | 0.848924 |
Target: 5'- -cGCGAAcgguucGGCUCCUCGGGgGCgccggGAGc -3' miRNA: 3'- caUGCUU------UUGAGGAGCCCgCGa----UUCa -5' |
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9605 | 3' | -50.8 | NC_002577.1 | + | 24958 | 1.08 | 0.011419 |
Target: 5'- cGUACGAAAACUCCUCGGGCGCUAAGUc -3' miRNA: 3'- -CAUGCUUUUGAGGAGCCCGCGAUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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