Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9606 | 5' | -58.1 | NC_002577.1 | + | 10437 | 0.66 | 0.850875 |
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Target: 5'- -cGAACCCCgaaaggggGGGUACgAGGaagCUUGCGGc -3' miRNA: 3'- cuCUUGGGG--------UCCAUG-UCCg--GAGCGCU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 123177 | 0.66 | 0.850875 |
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Target: 5'- -cGAACCCCgaaaggggGGGUACgAGGaagCUUGCGGc -3' miRNA: 3'- cuCUUGGGG--------UCCAUG-UCCg--GAGCGCU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 70881 | 0.68 | 0.707016 |
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Target: 5'- uGGAACCuCCGGG-ACAGaGaCCUCGCu- -3' miRNA: 3'- cUCUUGG-GGUCCaUGUC-C-GGAGCGcu -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 137426 | 0.69 | 0.657158 |
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Target: 5'- aGGAGCgCCGuGUACGcGGCCUaCGCGAa -3' miRNA: 3'- cUCUUGgGGUcCAUGU-CCGGA-GCGCU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 159911 | 0.69 | 0.657158 |
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Target: 5'- aGGAGCgCCGuGUACGcGGCCUaCGCGAa -3' miRNA: 3'- cUCUUGgGGUcCAUGU-CCGGA-GCGCU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 57811 | 0.71 | 0.576755 |
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Target: 5'- cGAGGcccGCCCUg---GCGGGCCUCGUGAa -3' miRNA: 3'- -CUCU---UGGGGuccaUGUCCGGAGCGCU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 57997 | 0.72 | 0.479917 |
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Target: 5'- cGAGGcCCgCCAGG-GCGGGCCUCGUuuGAg -3' miRNA: 3'- -CUCUuGG-GGUCCaUGUCCGGAGCG--CU- -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 28300 | 0.73 | 0.452386 |
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Target: 5'- cAGAAguucCCCCGGcGUGCuGGCCUCGUGu -3' miRNA: 3'- cUCUU----GGGGUC-CAUGuCCGGAGCGCu -5' |
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| 9606 | 5' | -58.1 | NC_002577.1 | + | 33470 | 1.08 | 0.00213 |
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Target: 5'- gGAGAACCCCAGGUACAGGCCUCGCGAa -3' miRNA: 3'- -CUCUUGGGGUCCAUGUCCGGAGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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