Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9607 | 5' | -47.3 | NC_002577.1 | + | 74498 | 0.65 | 0.999925 |
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Target: 5'- aGUCCUgCACGacgacagGGCUUacUCUGAg -3' miRNA: 3'- aCAGGA-GUGCaauuua-CCGAA--AGGCU- -5' |
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| 9607 | 5' | -47.3 | NC_002577.1 | + | 83823 | 0.66 | 0.999906 |
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Target: 5'- aUGUCCUCgcACGUgacGUGGaggUUCCa- -3' miRNA: 3'- -ACAGGAG--UGCAauuUACCga-AAGGcu -5' |
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| 9607 | 5' | -47.3 | NC_002577.1 | + | 49097 | 0.67 | 0.999579 |
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Target: 5'- -cUCCUUuagggACGgcGAAUGGCUaUCUGAa -3' miRNA: 3'- acAGGAG-----UGCaaUUUACCGAaAGGCU- -5' |
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| 9607 | 5' | -47.3 | NC_002577.1 | + | 32950 | 0.68 | 0.998517 |
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Target: 5'- aGUUCUCGCGU---AUGGCUgUuuGGc -3' miRNA: 3'- aCAGGAGUGCAauuUACCGAaAggCU- -5' |
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| 9607 | 5' | -47.3 | NC_002577.1 | + | 43464 | 0.69 | 0.996941 |
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Target: 5'- cUGUCCgCGCGUUGcu--GCUUUCCGu -3' miRNA: 3'- -ACAGGaGUGCAAUuuacCGAAAGGCu -5' |
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| 9607 | 5' | -47.3 | NC_002577.1 | + | 35529 | 1.02 | 0.05514 |
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Target: 5'- -uUCCUCACGUUAAAUGGCUUUCCGAc -3' miRNA: 3'- acAGGAGUGCAAUUUACCGAAAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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