Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9612 | 5' | -48.8 | NC_002577.1 | + | 33325 | 0.71 | 0.97607 |
Target: 5'- aGCGCucucGCGAGCGGcUCGUUAcGCGu -3' miRNA: 3'- -CGCGcauaCGUUCGCU-AGCAAUuCGC- -5' |
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9612 | 5' | -48.8 | NC_002577.1 | + | 22802 | 0.68 | 0.994947 |
Target: 5'- uGCGCGUGUaaggaGCcuGGCGGacgcaUCGgUGAGCGa -3' miRNA: 3'- -CGCGCAUA-----CGu-UCGCU-----AGCaAUUCGC- -5' |
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9612 | 5' | -48.8 | NC_002577.1 | + | 10267 | 0.68 | 0.996877 |
Target: 5'- aCGCGUAcau-AGCGAUCGUgggcUGGGCa -3' miRNA: 3'- cGCGCAUacguUCGCUAGCA----AUUCGc -5' |
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9612 | 5' | -48.8 | NC_002577.1 | + | 9301 | 0.67 | 0.997365 |
Target: 5'- uCGCGUacaGUGCGu-CGAUCGUUAAGg- -3' miRNA: 3'- cGCGCA---UACGUucGCUAGCAAUUCgc -5' |
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9612 | 5' | -48.8 | NC_002577.1 | + | 9270 | 0.69 | 0.988236 |
Target: 5'- cGUGCGUguacgagaccgcGUGCGAGCGGucUCGUcgUAGuGCGu -3' miRNA: 3'- -CGCGCA------------UACGUUCGCU--AGCA--AUU-CGC- -5' |
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9612 | 5' | -48.8 | NC_002577.1 | + | 7948 | 0.71 | 0.967332 |
Target: 5'- aGCGCGUAaGUAccggcguuccgGGCGGUCGgcGAGuCGg -3' miRNA: 3'- -CGCGCAUaCGU-----------UCGCUAGCaaUUC-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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