Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9613 | 3' | -61 | NC_002577.1 | + | 102464 | 0.66 | 0.707579 |
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Target: 5'- cGCgcaaUGCGCgCGCGgUagGGCCUCGUa-- -3' miRNA: 3'- -CGa---ACGCGgGCGCgG--CCGGAGCAaga -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 122109 | 0.67 | 0.658871 |
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Target: 5'- cCUUGCGUCCguucgcgggaaGCGCCGGaCCggCGcUCUa -3' miRNA: 3'- cGAACGCGGG-----------CGCGGCC-GGa-GCaAGA- -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 11505 | 0.67 | 0.658871 |
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Target: 5'- cCUUGCGUCCguucgcgggaaGCGCCGGaCCggCGcUCUa -3' miRNA: 3'- cGAACGCGGG-----------CGCGGCC-GGa-GCaAGA- -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 57994 | 0.67 | 0.639194 |
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Target: 5'- -----gGCCCGCcaggGCgGGCCUCGUUUg -3' miRNA: 3'- cgaacgCGGGCG----CGgCCGGAGCAAGa -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 153785 | 0.68 | 0.619497 |
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Target: 5'- --cUGCG-UCGCGCCGGCCgggcccCGUUUg -3' miRNA: 3'- cgaACGCgGGCGCGGCCGGa-----GCAAGa -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 11943 | 0.7 | 0.485314 |
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Target: 5'- ---cGCGCCCgguccGCGCCcuccGGCgUCGUUCUc -3' miRNA: 3'- cgaaCGCGGG-----CGCGG----CCGgAGCAAGA- -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 121671 | 0.7 | 0.485314 |
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Target: 5'- ---cGCGCCCgguccGCGCCcuccGGCgUCGUUCUc -3' miRNA: 3'- cgaaCGCGGG-----CGCGG----CCGgAGCAAGA- -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 163263 | 0.74 | 0.294829 |
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Target: 5'- aGCgaGCGCCCGCG-CGGCCggCGggCa -3' miRNA: 3'- -CGaaCGCGGGCGCgGCCGGa-GCaaGa -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 134065 | 0.74 | 0.294829 |
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Target: 5'- aGCgaGCGCCCGCG-CGGCCggCGggCa -3' miRNA: 3'- -CGaaCGCGGGCGCgGCCGGa-GCaaGa -5' |
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| 9613 | 3' | -61 | NC_002577.1 | + | 57851 | 1.09 | 0.001196 |
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Target: 5'- cGCUUGCGCCCGCGCCGGCCUCGUUCUu -3' miRNA: 3'- -CGAACGCGGGCGCGGCCGGAGCAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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