Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9615 | 5' | -55 | NC_002577.1 | + | 134099 | 0.68 | 0.841577 |
Target: 5'- cGGGGAUGCAGcGUgCGGCUCGGCc--- -3' miRNA: 3'- -CUCCUAUGUUcCG-GUCGAGCCGcuuu -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 163229 | 0.68 | 0.841577 |
Target: 5'- cGGGGAUGCAGcGUgCGGCUCGGCc--- -3' miRNA: 3'- -CUCCUAUGUUcCG-GUCGAGCCGcuuu -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 138492 | 0.69 | 0.797729 |
Target: 5'- aAGGGUACGAGGCCGGgggaGGcCGggGu -3' miRNA: 3'- cUCCUAUGUUCCGGUCgag-CC-GCuuU- -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 158846 | 0.69 | 0.797729 |
Target: 5'- aAGGGUACGAGGCCGGgggaGGcCGggGu -3' miRNA: 3'- cUCCUAUGUUCCGGUCgag-CC-GCuuU- -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 132053 | 0.7 | 0.730054 |
Target: 5'- -cGGAUugAGGGUCggGGC-CGGCGAGGg -3' miRNA: 3'- cuCCUAugUUCCGG--UCGaGCCGCUUU- -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 1561 | 0.7 | 0.730054 |
Target: 5'- -cGGAUugAGGGUCggGGC-CGGCGAGGg -3' miRNA: 3'- cuCCUAugUUCCGG--UCGaGCCGCUUU- -5' |
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9615 | 5' | -55 | NC_002577.1 | + | 59413 | 1.06 | 0.004805 |
Target: 5'- gGAGGAUACAAGGCCAGCUCGGCGAAAa -3' miRNA: 3'- -CUCCUAUGUUCCGGUCGAGCCGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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