Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9619 | 3' | -55.5 | NC_002577.1 | + | 126077 | 0.66 | 0.938315 |
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Target: 5'- cGGAC-AUCAcgcCGGGGUCGaACGcgGGAAa -3' miRNA: 3'- -UCUGaUGGU---GCCCCAGC-UGCa-CCUUa -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 138347 | 0.66 | 0.938315 |
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Target: 5'- --cCUGCCuCGcGGGUCGGCGaccUGGAu- -3' miRNA: 3'- ucuGAUGGuGC-CCCAGCUGC---ACCUua -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 7537 | 0.66 | 0.938315 |
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Target: 5'- cGGAC-AUCAcgcCGGGGUCGaACGcgGGAAa -3' miRNA: 3'- -UCUGaUGGU---GCCCCAGC-UGCa-CCUUa -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 158991 | 0.66 | 0.938315 |
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Target: 5'- --cCUGCCuCGcGGGUCGGCGaccUGGAu- -3' miRNA: 3'- ucuGAUGGuGC-CCCAGCUGC---ACCUua -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 143214 | 0.66 | 0.922932 |
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Target: 5'- aAGG--ACCAUGGGGUCG-CGUccGGGAg -3' miRNA: 3'- -UCUgaUGGUGCCCCAGCuGCA--CCUUa -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 55485 | 0.69 | 0.797841 |
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Target: 5'- --cCUAgUGCGGGGUCGAuccccCGUGGAGa -3' miRNA: 3'- ucuGAUgGUGCCCCAGCU-----GCACCUUa -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 551 | 0.7 | 0.770288 |
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Target: 5'- uGGCgguuaggGCCucuCGGGGUCGGCGUcaGGAc- -3' miRNA: 3'- uCUGa------UGGu--GCCCCAGCUGCA--CCUua -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 133063 | 0.7 | 0.770288 |
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Target: 5'- uGGCgguuaggGCCucuCGGGGUCGGCGUcaGGAc- -3' miRNA: 3'- uCUGa------UGGu--GCCCCAGCUGCA--CCUua -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 79175 | 0.74 | 0.520366 |
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Target: 5'- cAGACcgGCCGCGGGGUU--CGUGGAu- -3' miRNA: 3'- -UCUGa-UGGUGCCCCAGcuGCACCUua -5' |
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| 9619 | 3' | -55.5 | NC_002577.1 | + | 69696 | 1.05 | 0.00635 |
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Target: 5'- aAGACUACCACGGGGUCGACGUGGAAUc -3' miRNA: 3'- -UCUGAUGGUGCCCCAGCUGCACCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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