Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9621 | 3' | -50.8 | NC_002577.1 | + | 125437 | 0.69 | 0.954373 |
Target: 5'- cGGACaccggugcguccggGCGCUCGGC-CgGGcGAACGGCg -3' miRNA: 3'- -UCUG--------------UGCGAGCCGuGaCUaUUUGCUG- -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 143239 | 0.69 | 0.966458 |
Target: 5'- gAGACGCuCUCGGCGCaGAgcgucuACGAa -3' miRNA: 3'- -UCUGUGcGAGCCGUGaCUauu---UGCUg -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 75857 | 0.7 | 0.932562 |
Target: 5'- gGGACGCGCUCGauucguccGUGCUGG--AGCGAUc -3' miRNA: 3'- -UCUGUGCGAGC--------CGUGACUauUUGCUG- -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 45008 | 0.7 | 0.93767 |
Target: 5'- cGGCACGUUCGcGCGCUGcgGu-CGAUu -3' miRNA: 3'- uCUGUGCGAGC-CGUGACuaUuuGCUG- -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 12110 | 0.7 | 0.942523 |
Target: 5'- --uCGCGCUCGGCGCgccgGAGCaGGCg -3' miRNA: 3'- ucuGUGCGAGCCGUGacuaUUUG-CUG- -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 121587 | 0.74 | 0.780447 |
Target: 5'- -cGCGCGCUCGGCGCgccgGAGCaGGCg -3' miRNA: 3'- ucUGUGCGAGCCGUGacuaUUUG-CUG- -5' |
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9621 | 3' | -50.8 | NC_002577.1 | + | 74294 | 1.11 | 0.006937 |
Target: 5'- cAGACACGCUCGGCACUGAUAAACGACg -3' miRNA: 3'- -UCUGUGCGAGCCGUGACUAUUUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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