Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9625 | 5' | -53.5 | NC_002577.1 | + | 22812 | 0.66 | 0.966613 |
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Target: 5'- aGACUaucauUGCGCGUguaaGGAGCCUggcgGACGcaUCg -3' miRNA: 3'- -CUGG-----ACGUGCAg---UCUCGGAa---CUGU--AG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 152757 | 0.66 | 0.95588 |
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Target: 5'- gGACCUGCGCGUUuGAuGCaaaccGACAUUc -3' miRNA: 3'- -CUGGACGUGCAGuCU-CGgaa--CUGUAG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 58366 | 0.67 | 0.947547 |
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Target: 5'- cGCCUGCAuCGUCGGcGCgaUGuACAUUg -3' miRNA: 3'- cUGGACGU-GCAGUCuCGgaAC-UGUAG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 101518 | 0.67 | 0.933206 |
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Target: 5'- gGACCcGCACagaugCGGAGCCUUcgcccGCAUCg -3' miRNA: 3'- -CUGGaCGUGca---GUCUCGGAAc----UGUAG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 56474 | 0.67 | 0.927926 |
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Target: 5'- --aCUGUAcCGUCGGAGCCguucUGGCAc- -3' miRNA: 3'- cugGACGU-GCAGUCUCGGa---ACUGUag -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 158264 | 0.73 | 0.652157 |
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Target: 5'- gGGCC-GCACGUCGGGGCCgacGAUguaGUCg -3' miRNA: 3'- -CUGGaCGUGCAGUCUCGGaa-CUG---UAG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 139074 | 0.73 | 0.652157 |
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Target: 5'- gGGCC-GCACGUCGGGGCCgacGAUguaGUCg -3' miRNA: 3'- -CUGGaCGUGCAGUCUCGGaa-CUG---UAG- -5' |
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| 9625 | 5' | -53.5 | NC_002577.1 | + | 85709 | 1.1 | 0.003829 |
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Target: 5'- gGACCUGCACGUCAGAGCCUUGACAUCg -3' miRNA: 3'- -CUGGACGUGCAGUCUCGGAACUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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