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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9627 | 3' | -55.8 | NC_002577.1 | + | 112810 | 0.66 | 0.88992 |
Target: 5'- cGAGACCGGgcuuauaucAGGcCGCCUGCUUCc-- -3' miRNA: 3'- -CUCUGGUCac-------UUC-GCGGACGGAGauu -5' |
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9627 | 3' | -55.8 | NC_002577.1 | + | 49203 | 0.67 | 0.818895 |
Target: 5'- --uGCCGGUGAAGaguCGCCU-CCUCUGGu -3' miRNA: 3'- cucUGGUCACUUC---GCGGAcGGAGAUU- -5' |
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9627 | 3' | -55.8 | NC_002577.1 | + | 101655 | 1.06 | 0.003792 |
Target: 5'- cGAGACCAGUGAAGCGCCUGCCUCUAAu -3' miRNA: 3'- -CUCUGGUCACUUCGCGGACGGAGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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