Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9628 | 3' | -53.5 | NC_002577.1 | + | 16584 | 0.66 | 0.980336 |
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Target: 5'- -gGAGGUgGGGACCgucccgauuaAGGUCaaugccUGGACGc -3' miRNA: 3'- agCUCCAgCUCUGGa---------UCCAG------AUCUGC- -5' |
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| 9628 | 3' | -53.5 | NC_002577.1 | + | 16189 | 0.66 | 0.969365 |
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Target: 5'- gCGGGGUCGAGACaucguccgauucgccAGGUCUcGGAa- -3' miRNA: 3'- aGCUCCAGCUCUGga-------------UCCAGA-UCUgc -5' |
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| 9628 | 3' | -53.5 | NC_002577.1 | + | 151662 | 0.67 | 0.964008 |
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Target: 5'- -aGAGGUgGAGucGCCcuuguagauuuUGGGUUUGGGCGu -3' miRNA: 3'- agCUCCAgCUC--UGG-----------AUCCAGAUCUGC- -5' |
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| 9628 | 3' | -53.5 | NC_002577.1 | + | 40285 | 0.71 | 0.834978 |
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Target: 5'- gCGA-GUCGGGuCCUGcGGUCUAGAUa -3' miRNA: 3'- aGCUcCAGCUCuGGAU-CCAGAUCUGc -5' |
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| 9628 | 3' | -53.5 | NC_002577.1 | + | 104907 | 0.72 | 0.791438 |
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Target: 5'- cCGAcGUCuAGACCUAGGUCUcGACc -3' miRNA: 3'- aGCUcCAGcUCUGGAUCCAGAuCUGc -5' |
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| 9628 | 3' | -53.5 | NC_002577.1 | + | 105101 | 1.09 | 0.005798 |
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Target: 5'- aUCGAGGUCGAGACCUAGGUCUAGACGu -3' miRNA: 3'- -AGCUCCAGCUCUGGAUCCAGAUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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