Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9629 | 5' | -52.8 | NC_002577.1 | + | 112100 | 0.66 | 0.974119 |
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Target: 5'- cACGGGCcaug---CUGCGGAacuGCCGUAc -3' miRNA: 3'- -UGCUCGcauauaaGACGCCU---CGGCAU- -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 111947 | 0.66 | 0.971312 |
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Target: 5'- aAUGAGUGccuggAUGUUCUaGCGGcaGGCCGg- -3' miRNA: 3'- -UGCUCGCa----UAUAAGA-CGCC--UCGGCau -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 37540 | 0.67 | 0.965055 |
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Target: 5'- -gGAGCGgcUG---UGCGGAuGCCGUGg -3' miRNA: 3'- ugCUCGCauAUaagACGCCU-CGGCAU- -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 11175 | 0.69 | 0.907473 |
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Target: 5'- cGCGAGCGgagg-UCcgGCGGGGaCCGUc -3' miRNA: 3'- -UGCUCGCauauaAGa-CGCCUC-GGCAu -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 122439 | 0.69 | 0.907473 |
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Target: 5'- cGCGAGCGgagg-UCcgGCGGGGaCCGUc -3' miRNA: 3'- -UGCUCGCauauaAGa-CGCCUC-GGCAu -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 74118 | 0.69 | 0.894391 |
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Target: 5'- cCGAGCGUG---UCUGUgaccauGGGGCCGUu -3' miRNA: 3'- uGCUCGCAUauaAGACG------CCUCGGCAu -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 89505 | 0.69 | 0.894391 |
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Target: 5'- cGCGAGagcaucCGUGUAUaucUCaUGCGGAGUCGUu -3' miRNA: 3'- -UGCUC------GCAUAUA---AG-ACGCCUCGGCAu -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 35967 | 0.7 | 0.880343 |
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Target: 5'- aACGAGCGUuUAg---GUGcGAGCCGUAa -3' miRNA: 3'- -UGCUCGCAuAUaagaCGC-CUCGGCAU- -5' |
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| 9629 | 5' | -52.8 | NC_002577.1 | + | 107544 | 1.07 | 0.00759 |
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Target: 5'- aACGAGCGUAUAUUCUGCGGAGCCGUAg -3' miRNA: 3'- -UGCUCGCAUAUAAGACGCCUCGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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