Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9638 | 5' | -51.3 | NC_002577.1 | + | 160523 | 0.66 | 0.995035 |
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Target: 5'- cGGGUUCUcgacggCGUuGuccGCGCCGuCGAGa -3' miRNA: 3'- -CCCAAGAaa----GCAuCu--CGCGGC-GUUCc -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 136814 | 0.66 | 0.995035 |
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Target: 5'- cGGGUUCUcgacggCGUuGuccGCGCCGuCGAGa -3' miRNA: 3'- -CCCAAGAaa----GCAuCu--CGCGGC-GUUCc -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 158571 | 0.68 | 0.981649 |
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Target: 5'- aGGUgUCUgaaUCGgcGuAGCGCCGCGGcGGu -3' miRNA: 3'- cCCA-AGAa--AGCauC-UCGCGGCGUU-CC- -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 159940 | 0.68 | 0.981649 |
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Target: 5'- cGGUUCggcuccUCG-GGGGCGCCGgGAGc -3' miRNA: 3'- cCCAAGaa----AGCaUCUCGCGGCgUUCc -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 138766 | 0.68 | 0.981649 |
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Target: 5'- aGGUgUCUgaaUCGgcGuAGCGCCGCGGcGGu -3' miRNA: 3'- cCCA-AGAa--AGCauC-UCGCGGCGUU-CC- -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 42600 | 0.69 | 0.958761 |
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Target: 5'- aGGUUCgcgucgcaCGUAGAaaGC-CCGCAGGGg -3' miRNA: 3'- cCCAAGaaa-----GCAUCU--CGcGGCGUUCC- -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 76677 | 0.7 | 0.941996 |
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Target: 5'- aGGGgaCUUUUGgucgAGAGCGCaGCcGGGu -3' miRNA: 3'- -CCCaaGAAAGCa---UCUCGCGgCGuUCC- -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 16694 | 0.72 | 0.896749 |
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Target: 5'- aGGGUUCUaguuaaGUAG-GCGCCGCGc-- -3' miRNA: 3'- -CCCAAGAaag---CAUCuCGCGGCGUucc -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 123254 | 1.15 | 0.004244 |
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Target: 5'- cGGGUUCUUUCGUAGAGCGCCGCAAGGg -3' miRNA: 3'- -CCCAAGAAAGCAUCUCGCGGCGUUCC- -5' |
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| 9638 | 5' | -51.3 | NC_002577.1 | + | 10360 | 1.15 | 0.004244 |
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Target: 5'- cGGGUUCUUUCGUAGAGCGCCGCAAGGg -3' miRNA: 3'- -CCCAAGAAAGCAUCUCGCGGCGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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