Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9640 | 5' | -70.1 | NC_002577.1 | + | 156682 | 0.66 | 0.325268 |
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Target: 5'- --cUACCCCCCUCCcucuacgcagacauCCUCCCCGUCu- -3' miRNA: 3'- uguGUGGGGGGGGG--------------GGGGGGGCGGua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 7446 | 0.66 | 0.327302 |
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Target: 5'- aGCAUGCCCUguaCCCCaCCCCCCUuCUAa -3' miRNA: 3'- -UGUGUGGGGg--GGGG-GGGGGGGcGGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 50953 | 0.66 | 0.327302 |
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Target: 5'- uCGCGCCCCCCauucgCCCUUCUCCCGagGUa -3' miRNA: 3'- uGUGUGGGGGG-----GGGGGGGGGGCggUA- -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 126168 | 0.66 | 0.327302 |
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Target: 5'- aGCAUGCCCUguaCCCCaCCCCCCUuCUAa -3' miRNA: 3'- -UGUGUGGGGg--GGGG-GGGGGGGcGGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 155835 | 0.66 | 0.33415 |
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Target: 5'- cGCAUcgcuucuuGCCCCCCgCCCaacaccaccaCCaCCCCGCCc- -3' miRNA: 3'- -UGUG--------UGGGGGGgGGG----------GG-GGGGCGGua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 141503 | 0.66 | 0.334151 |
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Target: 5'- cGCAUcgcuucuuGCCCCCCgCCCaacaccaccaCCaCCCCGCCc- -3' miRNA: 3'- -UGUG--------UGGGGGGgGGG----------GG-GGGGCGGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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