Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9640 | 5' | -70.1 | NC_002577.1 | + | 158121 | 0.69 | 0.193523 |
|
Target: 5'- -aAUGCCCCCCCCgCCCgCCaCCGCg-- -3' miRNA: 3'- ugUGUGGGGGGGGgGGG-GG-GGCGgua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 158439 | 0.68 | 0.226692 |
|
Target: 5'- -aGCGCCCauguuUCCCCCCCUUCCCGuuCCAg -3' miRNA: 3'- ugUGUGGG-----GGGGGGGGGGGGGC--GGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 160415 | 0.73 | 0.1042 |
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Target: 5'- uGCACAaggCCCCCCaCCCCCCCUgaagcgugccgcgaCGCCGg -3' miRNA: 3'- -UGUGUg--GGGGGG-GGGGGGGG--------------GCGGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 162959 | 0.84 | 0.013977 |
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Target: 5'- cAC-CACCCCCCCCCCCCCCCCGa--- -3' miRNA: 3'- -UGuGUGGGGGGGGGGGGGGGGCggua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 163157 | 0.9 | 0.005331 |
|
Target: 5'- uCGCGCCCCCCCCCCCCCCCC-CCGa -3' miRNA: 3'- uGUGUGGGGGGGGGGGGGGGGcGGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 164047 | 0.72 | 0.130177 |
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Target: 5'- -aAUAUCCCCCCCugcauuucaCCCCCCCCcCCAa -3' miRNA: 3'- ugUGUGGGGGGGG---------GGGGGGGGcGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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