Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9640 | 5' | -70.1 | NC_002577.1 | + | 139836 | 0.74 | 0.090775 |
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Target: 5'- aACGaguGCCCgCUCCCCCUCCCCGCCc- -3' miRNA: 3'- -UGUg--UGGGgGGGGGGGGGGGGCGGua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 123670 | 0.74 | 0.086465 |
|
Target: 5'- cCACACCCCUCCaccgaccaugCCCCaCCCCCGCUc- -3' miRNA: 3'- uGUGUGGGGGGG----------GGGG-GGGGGCGGua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 140909 | 0.75 | 0.071102 |
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Target: 5'- gGCGUGCCCCCCCCaCCCCCCC-CCGa -3' miRNA: 3'- -UGUGUGGGGGGGGgGGGGGGGcGGUa -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 1731 | 0.75 | 0.069376 |
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Target: 5'- -aGCgACCCucuCCCCCCCCCCCCC-CCAUu -3' miRNA: 3'- ugUG-UGGG---GGGGGGGGGGGGGcGGUA- -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 140704 | 0.76 | 0.056533 |
|
Target: 5'- cGCAUACCCCCCCuccgcacgguucccCUCCCCCCaCGCCu- -3' miRNA: 3'- -UGUGUGGGGGGG--------------GGGGGGGG-GCGGua -5' |
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| 9640 | 5' | -70.1 | NC_002577.1 | + | 125307 | 1.04 | 0.000451 |
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Target: 5'- cACACACCCCCCCCCCCCCCCCGCCAUc -3' miRNA: 3'- -UGUGUGGGGGGGGGGGGGGGGCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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