Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9643 | 5' | -50.5 | NC_002577.1 | + | 67248 | 0.67 | 0.985207 |
Target: 5'- --cCCGGUgaucuuaacGCUUUUCCCUacaUCCAUg -3' miRNA: 3'- aaaGGCCAaa-------CGGAAAGGGA---AGGUAg -5' |
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9643 | 5' | -50.5 | NC_002577.1 | + | 6825 | 0.8 | 0.446908 |
Target: 5'- -gUuuGGUgUGUUUUUCCCUUCCAUCg -3' miRNA: 3'- aaAggCCAaACGGAAAGGGAAGGUAG- -5' |
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9643 | 5' | -50.5 | NC_002577.1 | + | 126788 | 0.8 | 0.446908 |
Target: 5'- -gUuuGGUgUGUUUUUCCCUUCCAUCg -3' miRNA: 3'- aaAggCCAaACGGAAAGGGAAGGUAG- -5' |
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9643 | 5' | -50.5 | NC_002577.1 | + | 126942 | 1.09 | 0.008948 |
Target: 5'- uUUUCCGGUUUGCCUUUCCCUUCCAUCg -3' miRNA: 3'- -AAAGGCCAAACGGAAAGGGAAGGUAG- -5' |
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9643 | 5' | -50.5 | NC_002577.1 | + | 6672 | 1.09 | 0.008948 |
Target: 5'- uUUUCCGGUUUGCCUUUCCCUUCCAUCg -3' miRNA: 3'- -AAAGGCCAAACGGAAAGGGAAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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