Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9644 | 3' | -49.9 | NC_002577.1 | + | 86038 | 0.66 | 0.995086 |
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Target: 5'- --aUCGGUCuggUCUCUGGCGguaccaUCUGcACGg -3' miRNA: 3'- uaaAGUCAG---AGAGACCGU------AGAC-UGUu -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 104463 | 0.68 | 0.97833 |
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Target: 5'- ---aCGGUCcaCUUUGGCAUuCUGACAGc -3' miRNA: 3'- uaaaGUCAGa-GAGACCGUA-GACUGUU- -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 5726 | 0.68 | 0.972961 |
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Target: 5'- ---cCAGUCUgUUUUGGCAUCUGAg-- -3' miRNA: 3'- uaaaGUCAGA-GAGACCGUAGACUguu -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 127887 | 0.68 | 0.972961 |
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Target: 5'- ---cCAGUCUgUUUUGGCAUCUGAg-- -3' miRNA: 3'- uaaaGUCAGA-GAGACCGUAGACUguu -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 104089 | 0.7 | 0.946689 |
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Target: 5'- ---aCGG-CUCUCUGGCGUaCUGGCc- -3' miRNA: 3'- uaaaGUCaGAGAGACCGUA-GACUGuu -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 128112 | 1.04 | 0.017404 |
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Target: 5'- cAUUUCAGUCUCUCUGGCAUCUGACAAc -3' miRNA: 3'- -UAAAGUCAGAGAGACCGUAGACUGUU- -5' |
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| 9644 | 3' | -49.9 | NC_002577.1 | + | 5502 | 1.04 | 0.017404 |
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Target: 5'- cAUUUCAGUCUCUCUGGCAUCUGACAAc -3' miRNA: 3'- -UAAAGUCAGAGAGACCGUAGACUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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