Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9650 | 3' | -55.6 | NC_002577.1 | + | 132576 | 0.68 | 0.83784 |
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Target: 5'- uUUCGcgGGg--GGcUGGCGGAGACCg -3' miRNA: 3'- -AAGCuaCCaggCCaACUGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 1037 | 0.68 | 0.83784 |
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Target: 5'- uUUCGcgGGg--GGcUGGCGGAGACCg -3' miRNA: 3'- -AAGCuaCCaggCCaACUGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 158489 | 0.68 | 0.829546 |
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Target: 5'- uUUCGAUGG-CCGGaccgcaGGCGGGGaACCc -3' miRNA: 3'- -AAGCUACCaGGCCaa----CUGCCUC-UGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 138848 | 0.68 | 0.829546 |
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Target: 5'- uUUCGAUGG-CCGGaccgcaGGCGGGGaACCc -3' miRNA: 3'- -AAGCUACCaGGCCaa----CUGCCUC-UGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 125071 | 0.7 | 0.728303 |
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Target: 5'- cUCGAaugUGGaaUUCGGUUccGACGGGGGCCg -3' miRNA: 3'- aAGCU---ACC--AGGCCAA--CUGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 8543 | 0.7 | 0.728303 |
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Target: 5'- cUCGAaugUGGaaUUCGGUUccGACGGGGGCCg -3' miRNA: 3'- aAGCU---ACC--AGGCCAA--CUGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 156315 | 0.72 | 0.657881 |
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Target: 5'- gUCGGggGGUUCGGcgGaACGGAGACCc -3' miRNA: 3'- aAGCUa-CCAGGCCaaC-UGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 141022 | 0.72 | 0.657881 |
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Target: 5'- gUCGGggGGUUCGGcgGaACGGAGACCc -3' miRNA: 3'- aAGCUa-CCAGGCCaaC-UGCCUCUGGa -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 129525 | 0.91 | 0.050466 |
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Target: 5'- cUUCGAUGGUCCGGUguACGGAGACCUc -3' miRNA: 3'- -AAGCUACCAGGCCAacUGCCUCUGGA- -5' |
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| 9650 | 3' | -55.6 | NC_002577.1 | + | 4088 | 0.91 | 0.050466 |
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Target: 5'- cUUCGAUGGUCCGGUguACGGAGACCUc -3' miRNA: 3'- -AAGCUACCAGGCCAacUGCCUCUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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