Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9653 | 5' | -51.8 | NC_002577.1 | + | 130361 | 0.96 | 0.046601 |
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Target: 5'- gACCUCUCACACACAGCACAAAG-ACGg -3' miRNA: 3'- -UGGAGAGUGUGUGUCGUGUUUCcUGC- -5' |
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| 9653 | 5' | -51.8 | NC_002577.1 | + | 132120 | 0.68 | 0.966791 |
|
Target: 5'- uGCg-CUCGCGCGCgAGC-CAGAcGGACGg -3' miRNA: 3'- -UGgaGAGUGUGUG-UCGuGUUU-CCUGC- -5' |
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| 9653 | 5' | -51.8 | NC_002577.1 | + | 135638 | 0.75 | 0.682302 |
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Target: 5'- gGCCUCUgGCGCcCGGcCACAAGGGcCGa -3' miRNA: 3'- -UGGAGAgUGUGuGUC-GUGUUUCCuGC- -5' |
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| 9653 | 5' | -51.8 | NC_002577.1 | + | 138946 | 0.7 | 0.900384 |
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Target: 5'- cGCCgauUCAgACACcuccgucagccguccGGCGCGAGGGACGa -3' miRNA: 3'- -UGGag-AGUgUGUG---------------UCGUGUUUCCUGC- -5' |
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| 9653 | 5' | -51.8 | NC_002577.1 | + | 158392 | 0.7 | 0.900384 |
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Target: 5'- cGCCgauUCAgACACcuccgucagccguccGGCGCGAGGGACGa -3' miRNA: 3'- -UGGag-AGUgUGUG---------------UCGUGUUUCCUGC- -5' |
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| 9653 | 5' | -51.8 | NC_002577.1 | + | 161700 | 0.75 | 0.682302 |
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Target: 5'- gGCCUCUgGCGCcCGGcCACAAGGGcCGa -3' miRNA: 3'- -UGGAGAgUGUGuGUC-GUGUUUCCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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