Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
9654 | 3' | -59.8 | NC_002577.1 | + | 97551 | 0.66 | 0.735372 |
Target: 5'- -cCCAGCACGGacGCcgaggCGACGC-GCGAa -3' miRNA: 3'- gaGGUCGUGCCa-CGa----GCUGCGcCGUU- -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 143150 | 0.66 | 0.7199 |
Target: 5'- uUCCGcGCugGGUGCUCGccgcACGCcccggucccacacuaGGCGc -3' miRNA: 3'- gAGGU-CGugCCACGAGC----UGCG---------------CCGUu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 159784 | 0.68 | 0.636444 |
Target: 5'- cCUCUAGCGCGGaauugaagGCcgCGACGCcccGGCGc -3' miRNA: 3'- -GAGGUCGUGCCa-------CGa-GCUGCG---CCGUu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 137554 | 0.68 | 0.636444 |
Target: 5'- cCUCUAGCGCGGaauugaagGCcgCGACGCcccGGCGc -3' miRNA: 3'- -GAGGUCGUGCCa-------CGa-GCUGCG---CCGUu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 4515 | 0.68 | 0.596328 |
Target: 5'- uUUCAGguCGGUGCuguuauuuUCGGCGgGGCGc -3' miRNA: 3'- gAGGUCguGCCACG--------AGCUGCgCCGUu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 129099 | 0.68 | 0.596328 |
Target: 5'- uUUCAGguCGGUGCuguuauuuUCGGCGgGGCGc -3' miRNA: 3'- gAGGUCguGCCACG--------AGCUGCgCCGUu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 38336 | 0.71 | 0.452469 |
Target: 5'- cCUCUGGCGCGG-GCg-GACGCGGUu- -3' miRNA: 3'- -GAGGUCGUGCCaCGagCUGCGCCGuu -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 130534 | 1.06 | 0.002114 |
Target: 5'- cCUCCAGCACGGUGCUCGACGCGGCAAu -3' miRNA: 3'- -GAGGUCGUGCCACGAGCUGCGCCGUU- -5' |
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9654 | 3' | -59.8 | NC_002577.1 | + | 3079 | 1.06 | 0.002114 |
Target: 5'- cCUCCAGCACGGUGCUCGACGCGGCAAu -3' miRNA: 3'- -GAGGUCGUGCCACGAGCUGCGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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