Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 966 | 3' | -55.2 | NC_000902.1 | + | 49882 | 0.66 | 0.739563 |
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Target: 5'- gCGCCCGGACaaccguuaUucacggaugagaaaAGCGCCAugGGGAGuGa -3' miRNA: 3'- -GUGGGUCUG--------G--------------UCGCGGUugUUCUCuC- -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 28473 | 0.67 | 0.645268 |
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Target: 5'- uCAgCCAGGacguCCAGCGUCAGCAAuGAGc- -3' miRNA: 3'- -GUgGGUCU----GGUCGCGGUUGUU-CUCuc -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 37597 | 0.68 | 0.612179 |
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Target: 5'- aGgCCAGGCUGGCGCaggagAGCGccGGAGAGa -3' miRNA: 3'- gUgGGUCUGGUCGCGg----UUGU--UCUCUC- -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 14094 | 0.68 | 0.601172 |
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Target: 5'- cCAUCUuaGGGCgAGCGCCAaugACAaacAGAGAGc -3' miRNA: 3'- -GUGGG--UCUGgUCGCGGU---UGU---UCUCUC- -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 50172 | 0.69 | 0.557494 |
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Target: 5'- uGCCCGuGGCaacGCGCCGACGGcAGAGg -3' miRNA: 3'- gUGGGU-CUGgu-CGCGGUUGUUcUCUC- -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 51600 | 0.69 | 0.557494 |
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Target: 5'- --aCCGGACCAGaaCGCCG-CAAGGGGa -3' miRNA: 3'- gugGGUCUGGUC--GCGGUuGUUCUCUc -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 12179 | 0.7 | 0.463358 |
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Target: 5'- uUugCCAGACCgcgcuggguaAGCGUCGACAGGuugauGAGu -3' miRNA: 3'- -GugGGUCUGG----------UCGCGGUUGUUCu----CUC- -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 6260 | 0.71 | 0.424235 |
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Target: 5'- -cCCCAGGCCAGUGCUuuAGCGGG-GAu -3' miRNA: 3'- guGGGUCUGGUCGCGG--UUGUUCuCUc -5' |
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| 966 | 3' | -55.2 | NC_000902.1 | + | 45949 | 1.09 | 0.001015 |
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Target: 5'- gCACCCAGACCAGCGCCAACAAGAGAGg -3' miRNA: 3'- -GUGGGUCUGGUCGCGGUUGUUCUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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