Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 966 | 5' | -50.8 | NC_000902.1 | + | 36815 | 0.66 | 0.910623 |
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Target: 5'- aUGUUGUUUCGcgcuuucaguaaGGAUGCUGCCgGUGg -3' miRNA: 3'- -ACGACAAAGCa-----------UUUGCGGCGGaCACg -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 27278 | 0.67 | 0.905214 |
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Target: 5'- cGCUGg-UCGUAAGCGCCugcggaucaacGCCaccUGCu -3' miRNA: 3'- aCGACaaAGCAUUUGCGG-----------CGGac-ACG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 7162 | 0.67 | 0.898195 |
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Target: 5'- cGCUGgcaUCaccuuUAAAUGCCGUCUG-GCg -3' miRNA: 3'- aCGACaa-AGc----AUUUGCGGCGGACaCG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 22927 | 0.67 | 0.898195 |
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Target: 5'- cGCUGaggUCGUcGGACgGuuGCCUG-GCu -3' miRNA: 3'- aCGACaa-AGCA-UUUG-CggCGGACaCG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 36976 | 0.67 | 0.890893 |
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Target: 5'- uUGUUGUUUCGggGACGCUGaaaucuCCUGaUGg -3' miRNA: 3'- -ACGACAAAGCauUUGCGGC------GGAC-ACg -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 48523 | 0.67 | 0.875459 |
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Target: 5'- gGCUGac-CGguaAGAUGUCGCCggGUGCa -3' miRNA: 3'- aCGACaaaGCa--UUUGCGGCGGa-CACG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 55282 | 0.69 | 0.793809 |
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Target: 5'- cGUcaUGuUUUUGgcaagAGACGCCGCCUGcugGCg -3' miRNA: 3'- aCG--AC-AAAGCa----UUUGCGGCGGACa--CG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 59587 | 0.71 | 0.675455 |
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Target: 5'- gGCUG-UUCGUGGAUGCagaGCUUGcGCc -3' miRNA: 3'- aCGACaAAGCAUUUGCGg--CGGACaCG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 31011 | 0.78 | 0.310267 |
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Target: 5'- gGCUucagCGUGAACGCCGUCgUGUGCu -3' miRNA: 3'- aCGAcaaaGCAUUUGCGGCGG-ACACG- -5' |
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| 966 | 5' | -50.8 | NC_000902.1 | + | 45914 | 1.13 | 0.001547 |
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Target: 5'- cUGCUGUUUCGUAAACGCCGCCUGUGCa -3' miRNA: 3'- -ACGACAAAGCAUUUGCGGCGGACACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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