Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9660 | 5' | -62.5 | NC_002577.1 | + | 77505 | 0.66 | 0.629887 |
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Target: 5'- -uGCGGGCAUuCGCGUGGaaaCGGGCgCg -3' miRNA: 3'- auCGCUCGUAcGCGCGCC---GCCUGgGa -5' |
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| 9660 | 5' | -62.5 | NC_002577.1 | + | 35981 | 0.69 | 0.438781 |
|
Target: 5'- -uGCGAGcCGUaaaaaugaaugccucGCGCGCGGCGGGaguuuagucgacgccCCCUu -3' miRNA: 3'- auCGCUC-GUA---------------CGCGCGCCGCCU---------------GGGA- -5' |
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| 9660 | 5' | -62.5 | NC_002577.1 | + | 16933 | 0.68 | 0.503875 |
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Target: 5'- -cGUGAGaCGcucGCuGCGCGGCaGGGCCCa -3' miRNA: 3'- auCGCUC-GUa--CG-CGCGCCG-CCUGGGa -5' |
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| 9660 | 5' | -62.5 | NC_002577.1 | + | 8492 | 0.72 | 0.287755 |
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Target: 5'- -uGUGGGUGUGCGCGCGGCcgGGACg-- -3' miRNA: 3'- auCGCUCGUACGCGCGCCG--CCUGgga -5' |
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| 9660 | 5' | -62.5 | NC_002577.1 | + | 7919 | 0.66 | 0.617041 |
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Target: 5'- cGGCGAGUcgGCGgagaaaagguggauCGCGaGCGcGAUCCg -3' miRNA: 3'- aUCGCUCGuaCGC--------------GCGC-CGC-CUGGGa -5' |
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| 9660 | 5' | -62.5 | NC_002577.1 | + | 7825 | 0.68 | 0.494571 |
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Target: 5'- cGGCGcuucuGCGUcUGCGCGGCGGACauaCg -3' miRNA: 3'- aUCGCu----CGUAcGCGCGCCGCCUGg--Ga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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