Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
967 | 3' | -54.4 | NC_000902.1 | + | 27141 | 0.66 | 0.766674 |
Target: 5'- cCAGCcACAGCGCaCcAUAGccaACCGCCAg -3' miRNA: 3'- -GUCGuUGUCGUG-GuUGUCc--UGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 7895 | 0.66 | 0.756302 |
Target: 5'- gGGCAGC--CAgCAACAGG-CCACCc -3' miRNA: 3'- gUCGUUGucGUgGUUGUCCuGGUGGu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 15362 | 0.66 | 0.755257 |
Target: 5'- aCGGUAauuGCAGUaaugaaaGCCAuCAGGGCCGCa- -3' miRNA: 3'- -GUCGU---UGUCG-------UGGUuGUCCUGGUGgu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 33707 | 0.66 | 0.745799 |
Target: 5'- aCAGCAGgaAGCGCCAaaaGCGGuuUCGCCGg -3' miRNA: 3'- -GUCGUUg-UCGUGGU---UGUCcuGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 32873 | 0.66 | 0.745799 |
Target: 5'- aAGCAACGG-GCCgAACAGGaaGCCGCa- -3' miRNA: 3'- gUCGUUGUCgUGG-UUGUCC--UGGUGgu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 45094 | 0.66 | 0.744742 |
Target: 5'- uCAGCAcugagcacagaacGCAGCACCAAaaa-GCCAUCAg -3' miRNA: 3'- -GUCGU-------------UGUCGUGGUUguccUGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 27154 | 0.66 | 0.724451 |
Target: 5'- aGGCGAaugcCACCAuuacugacauGCAGGugCGCCAg -3' miRNA: 3'- gUCGUUguc-GUGGU----------UGUCCugGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 48706 | 0.67 | 0.713629 |
Target: 5'- uCAGguACAagcGCACCAAuCAGGuuuacacccuucGCCACCc -3' miRNA: 3'- -GUCguUGU---CGUGGUU-GUCC------------UGGUGGu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 49389 | 0.67 | 0.702724 |
Target: 5'- cCGGCAACcGCACCGAauGGAgCCcCCAu -3' miRNA: 3'- -GUCGUUGuCGUGGUUguCCU-GGuGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 37024 | 0.67 | 0.702724 |
Target: 5'- gAGCAaauauuacccugACGGCGCUGACAGuuUCACCGg -3' miRNA: 3'- gUCGU------------UGUCGUGGUUGUCcuGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 38609 | 0.67 | 0.702724 |
Target: 5'- aCAGguGCGGCagGCCcgGugGGGGCgACCGg -3' miRNA: 3'- -GUCguUGUCG--UGG--UugUCCUGgUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 250 | 0.67 | 0.691749 |
Target: 5'- gGGCAcucCAGCGCCAGCAuGGuCUACa- -3' miRNA: 3'- gUCGUu--GUCGUGGUUGU-CCuGGUGgu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 38569 | 0.67 | 0.680714 |
Target: 5'- -uGCGGCAgGC-CCGGCAGGcCCACa- -3' miRNA: 3'- guCGUUGU-CGuGGUUGUCCuGGUGgu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 55722 | 0.68 | 0.647371 |
Target: 5'- cCGGCAGCacgGGaCGCCGuCAGGucacccGCCGCCAc -3' miRNA: 3'- -GUCGUUG---UC-GUGGUuGUCC------UGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 46031 | 0.68 | 0.636213 |
Target: 5'- uGGCAuUAGCGCCGGugcCAGGuguuuuagcACCGCCAc -3' miRNA: 3'- gUCGUuGUCGUGGUU---GUCC---------UGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 28407 | 0.68 | 0.621703 |
Target: 5'- uGGCugaGACAGCACCAAaacuucgugcugacCGGGaaagaaucaacGCCGCCAg -3' miRNA: 3'- gUCG---UUGUCGUGGUU--------------GUCC-----------UGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 28985 | 0.68 | 0.613896 |
Target: 5'- -cGUAGCGGUGCCAGCGGuGGCUAUa- -3' miRNA: 3'- guCGUUGUCGUGGUUGUC-CUGGUGgu -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 12933 | 0.68 | 0.613896 |
Target: 5'- aAGCAAUAcuGUACCAGCAG-AUUACCAg -3' miRNA: 3'- gUCGUUGU--CGUGGUUGUCcUGGUGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 32216 | 0.69 | 0.54763 |
Target: 5'- gGGCAcauGCAGCugCAcaACGGGAUaauGCCAg -3' miRNA: 3'- gUCGU---UGUCGugGU--UGUCCUGg--UGGU- -5' |
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967 | 3' | -54.4 | NC_000902.1 | + | 33746 | 0.7 | 0.526 |
Target: 5'- aAGCAcgaaGCAG-ACCAgcGCGGGugCGCCGg -3' miRNA: 3'- gUCGU----UGUCgUGGU--UGUCCugGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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