Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9674 | 5' | -58.3 | NC_002577.1 | + | 154748 | 0.69 | 0.645132 |
Target: 5'- cGAGCAguucguUGGCGuGGCCACGAGCUcuGUUUGc -3' miRNA: 3'- -UUCGU------GCCGU-CUGGUGCUCGG--CGAAC- -5' |
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9674 | 5' | -58.3 | NC_002577.1 | + | 226 | 0.72 | 0.439405 |
Target: 5'- gGGGUGCGGCAGcCCGCGGGCUGa--- -3' miRNA: 3'- -UUCGUGCCGUCuGGUGCUCGGCgaac -5' |
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9674 | 5' | -58.3 | NC_002577.1 | + | 134139 | 0.75 | 0.317392 |
Target: 5'- -cGCGCGGCGGACCcuaGCGAGCaUGCg-- -3' miRNA: 3'- uuCGUGCCGUCUGG---UGCUCG-GCGaac -5' |
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9674 | 5' | -58.3 | NC_002577.1 | + | 134164 | 0.75 | 0.317392 |
Target: 5'- -cGCGCGGCGGACCcuaGCGAGCaUGCg-- -3' miRNA: 3'- uuCGUGCCGUCUGG---UGCUCG-GCGaac -5' |
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9674 | 5' | -58.3 | NC_002577.1 | + | 162308 | 0.79 | 0.178221 |
Target: 5'- -cGCACGGCAGACCGCGGcCCGUa-- -3' miRNA: 3'- uuCGUGCCGUCUGGUGCUcGGCGaac -5' |
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9674 | 5' | -58.3 | NC_002577.1 | + | 134114 | 0.66 | 0.818501 |
Target: 5'- -cGCGCGGCGGACC-CuGGCCa---- -3' miRNA: 3'- uuCGUGCCGUCUGGuGcUCGGcgaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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