miRNA: 3'- uUCgUGUGGuGGUGUCuGAGA-CuGGG- -5'
miRNA: 3'- -UUCG-----UGUGGUGG--------------UGUCUGAGa--CUGGG- -5'
miRNA: 3'- -UUCG-----UGUGGUGG--------------UGUCUGAGa--CUGGG- -5'
miRNA: 3'- uuCGUGUggUGGugUCUGAGAcUGGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9683 | 5' | -55 | NC_002577.1 | + | 139121 | 0.66 | 0.926796 |
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Target: 5'- cGGgACGCC-CCACGG-CUCUcGuCCCg -3' miRNA: 3'- uUCgUGUGGuGGUGUCuGAGA-CuGGG- -5' |
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| 9683 | 5' | -55 | NC_002577.1 | + | 11237 | 0.66 | 0.930509 |
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Target: 5'- gGGGCuagaaACGCCGCCccccucccacggggGCGGAUUCgggGACCUc -3' miRNA: 3'- -UUCG-----UGUGGUGG--------------UGUCUGAGa--CUGGG- -5' |
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| 9683 | 5' | -55 | NC_002577.1 | + | 122377 | 0.66 | 0.930509 |
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Target: 5'- gGGGCuagaaACGCCGCCccccucccacggggGCGGAUUCgggGACCUc -3' miRNA: 3'- -UUCG-----UGUGGUGG--------------UGUCUGAGa--CUGGG- -5' |
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| 9683 | 5' | -55 | NC_002577.1 | + | 58612 | 0.66 | 0.932064 |
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Target: 5'- -cGCGCGuuGCCugGGACUUUaGCUCu -3' miRNA: 3'- uuCGUGUggUGGugUCUGAGAcUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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