Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9689 | 3' | -57.5 | NC_002577.1 | + | 159106 | 0.66 | 0.84818 |
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Target: 5'- gUCAUCCggCGGUgGCGGcGCagcaguuuCCGAGGAc -3' miRNA: 3'- -GGUAGGaaGCCA-CGCCaCG--------GGCUCUU- -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 138232 | 0.66 | 0.84818 |
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Target: 5'- gUCAUCCggCGGUgGCGGcGCagcaguuuCCGAGGAc -3' miRNA: 3'- -GGUAGGaaGCCA-CGCCaCG--------GGCUCUU- -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 3444 | 0.66 | 0.840235 |
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Target: 5'- gCCGUCCUUU-GUGCuGUGUgUGAGAGg -3' miRNA: 3'- -GGUAGGAAGcCACGcCACGgGCUCUU- -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 130170 | 0.66 | 0.840234 |
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Target: 5'- gCCGUCCUUU-GUGCuGUGUgUGAGAGg -3' miRNA: 3'- -GGUAGGAAGcCACGcCACGgGCUCUU- -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 105694 | 0.69 | 0.713247 |
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Target: 5'- aUAUCC-UCGGcaaucGCGG-GCCCGGGAu -3' miRNA: 3'- gGUAGGaAGCCa----CGCCaCGGGCUCUu -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 128736 | 0.69 | 0.703388 |
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Target: 5'- uCCGUCCUa--GcGUGGUGCCUGAGAu -3' miRNA: 3'- -GGUAGGAagcCaCGCCACGGGCUCUu -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 4878 | 0.73 | 0.457798 |
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Target: 5'- uCCGUCCUUa-GcGUGGUGCCUGAGAu -3' miRNA: 3'- -GGUAGGAAgcCaCGCCACGGGCUCUu -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 129100 | 0.73 | 0.457798 |
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Target: 5'- uCCGUCCaUCGGcGUGGUGCUCGAu-- -3' miRNA: 3'- -GGUAGGaAGCCaCGCCACGGGCUcuu -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 4514 | 0.73 | 0.457798 |
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Target: 5'- uCCGUCCaUCGGcGUGGUGCUCGAu-- -3' miRNA: 3'- -GGUAGGaAGCCaCGCCACGGGCUcuu -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 130396 | 1.08 | 0.002825 |
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Target: 5'- uCCAUCCUUCGGUGCGGUGCCCGAGAAg -3' miRNA: 3'- -GGUAGGAAGCCACGCCACGGGCUCUU- -5' |
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| 9689 | 3' | -57.5 | NC_002577.1 | + | 3217 | 1.08 | 0.002825 |
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Target: 5'- uCCAUCCUUCGGUGCGGUGCCCGAGAAg -3' miRNA: 3'- -GGUAGGAAGCCACGCCACGGGCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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