Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
9709 | 3' | -60.4 | NC_002577.1 | + | 122673 | 0.66 | 0.727552 |
Target: 5'- uACCgcGGuCCCgcgcuUUCGGACGgcGGGCCGGc -3' miRNA: 3'- uUGGa-CCuGGG-----AGGCCUGU--UCCGGCC- -5' |
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9709 | 3' | -60.4 | NC_002577.1 | + | 135837 | 0.66 | 0.746424 |
Target: 5'- cAGCCUccucGGCCCUuguggCCGGGCGccagAGGCCGc -3' miRNA: 3'- -UUGGAc---CUGGGA-----GGCCUGU----UCCGGCc -5' |
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9709 | 3' | -60.4 | NC_002577.1 | + | 160440 | 0.66 | 0.762174 |
Target: 5'- cGCaaaGGGCCCgcaCGGGCucgggagaacuugcGGGGCCGGg -3' miRNA: 3'- uUGga-CCUGGGag-GCCUG--------------UUCCGGCC- -5' |
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9709 | 3' | -60.4 | NC_002577.1 | + | 2570 | 0.66 | 0.762174 |
Target: 5'- --gUUGGGCCCUUagGGGCAggggaggcguuuagGGGUCGGg -3' miRNA: 3'- uugGACCUGGGAGg-CCUGU--------------UCCGGCC- -5' |
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9709 | 3' | -60.4 | NC_002577.1 | + | 19973 | 0.66 | 0.764922 |
Target: 5'- -cCCUGuGcgucauACCCUuugcuacguguaCCGuGACGGGGCCGGa -3' miRNA: 3'- uuGGAC-C------UGGGA------------GGC-CUGUUCCGGCC- -5' |
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9709 | 3' | -60.4 | NC_002577.1 | + | 136902 | 0.66 | 0.762174 |
Target: 5'- ----aGGGCCCgcaCGGGCucgggagaacuugcGGGGCCGGg -3' miRNA: 3'- uuggaCCUGGGag-GCCUG--------------UUCCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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