Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9709 | 3' | -60.4 | NC_002577.1 | + | 160366 | 0.7 | 0.49457 |
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Target: 5'- -cCCUcGGACgUUCCGGAaGAGGCCGc -3' miRNA: 3'- uuGGA-CCUGgGAGGCCUgUUCCGGCc -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 126684 | 0.72 | 0.406418 |
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Target: 5'- uGGCCggUGGAUgagUCgcgauggCCGGACGGGGCCGGa -3' miRNA: 3'- -UUGG--ACCUG---GGa------GGCCUGUUCCGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 6930 | 0.72 | 0.406418 |
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Target: 5'- uGGCCggUGGAUgagUCgcgauggCCGGACGGGGCCGGa -3' miRNA: 3'- -UUGG--ACCUG---GGa------GGCCUGUUCCGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 8180 | 0.72 | 0.381952 |
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Target: 5'- cACC-GGugCgUCCGGGCGcucGGCCGGg -3' miRNA: 3'- uUGGaCCugGgAGGCCUGUu--CCGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 125433 | 0.72 | 0.381952 |
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Target: 5'- cACC-GGugCgUCCGGGCGcucGGCCGGg -3' miRNA: 3'- uUGGaCCugGgAGGCCUGUu--CCGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 16172 | 1.08 | 0.001451 |
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Target: 5'- cAACCUGGACCCUCCGGACAAGGCCGGg -3' miRNA: 3'- -UUGGACCUGGGAGGCCUGUUCCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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